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Literature summary for 3.2.1.8 extracted from

  • Zhou, J.; Huang, H.; Meng, K.; Shi, P.; Wang, Y.; Luo, H.; Yang, P.; Bai, Y.; Zhou, Z.; Yao, B.
    Molecular and biochemical characterization of a novel xylanase from the symbiotic Sphingobacterium sp. TN19 (2009), Appl. Microbiol. Biotechnol., 85, 323-333.
    View publication on PubMed
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
gene xynA19, phylogenetic tree, expression of His-tagged XynA19 without the signal peptide sequence in Escherichia coli strains BL21 (DE3) and Top10 Sphingobacterium sp. TN19

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information Km for the recombinant enzyme is 17.9 mg/ml Sphingobacterium sp. TN19

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
beta-1,4-xylan + H2O Sphingobacterium sp. TN19
-
 D-xylose + beta-1,4-xylobiose + ?
-
 ?

Organism

Organism UniProt Comment Textmining
Sphingobacterium sp. TN19 D8L2Y2 a symbiotic bacterium isolated from the gut of Batocera horsfieldi larvae, gene xynA19
-

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged XynA19 from Escherichia coli strains Top10 and BL21(DE3) by ultrafiltration and nickel affinity chromatography Sphingobacterium sp. TN19

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
196.9
-
 purified recombinant enzyme Sphingobacterium sp. TN19

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-1,4-xylan + H2O
-
 Sphingobacterium sp. TN19 D-xylose + beta-1,4-xylobiose + ?
-
 ?
beta-1,4-xylan + H2O
-
 Sphingobacterium sp. TN19 D-xylose + beta-1,4-xylobiose + ? hydrolysis products comprise 20.1% xylose and 79.9% xylobiose ?

Subunits

Subunits Comment Organism
More XynA19 contains a complete GH10 catalytic domain of GH 10 from Ile38 to Ala381, structure homology modeling of the recombinant enzyme, overview Sphingobacterium sp. TN19

Synonyms

Synonyms Comment Organism
More XynA19 belongs to glycosyl hydrolase family 10, GH 10 Sphingobacterium sp. TN19
xylanase
-
 Sphingobacterium sp. TN19
XynA19
-
 Sphingobacterium sp. TN19

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45
-
-
 Sphingobacterium sp. TN19

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
10 65 the enzyme shows over 65% of its maximum activity at 20-28°C and about 40% at 10°C Sphingobacterium sp. TN19

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
10
-
 purified recombinant enzyme, pH 6.5, 40% remaining activity Sphingobacterium sp. TN19
20
-
 purified recombinant enzyme, pH 6.5, 65% remaining activity Sphingobacterium sp. TN19
30 50 purified recombinant enzyme, pH 6.5, over 80% remaining activity, above 50°C the enzyme activity decreases rapidly after 10 min preincubation Sphingobacterium sp. TN19

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
603.8
-
 beta-1,4-xylan pH 6.5, 45°C Sphingobacterium sp. TN19

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.5
-
-
 Sphingobacterium sp. TN19