Cloned (Comment) | Organism |
---|---|
amplified fragment of Xyn11 (nucleotides 64-646) cloned into the pSE420-CHis vector and transformed into Escherichia coli strain XL1-Blue MR | uncultured bacterium |
Crystallization (Comment) | Organism |
---|---|
wild-type Xyn11 and hyperthermostable mutant Xyn11TS crystallized to a resolution of 1.8 and 1.9 A, respectively. Crystal structure of Xyn11TS is extremely similar to the wild-type. Crystals of Xyn11TS belong to space group C2, with unit cell dimensions of a=64.7 A, b=33.6 A, c=83.1 A, alpha=90.0°, beta=101.9°, gamma=90.0°, and with one molecule occupying the crystallographic asymmetric unit. Crystals of Xyn11 belong to space group P212121, with unit cell dimensions of a=36.3 A, b=63.2 A, c=75.1 A, alpha=beta=gamma=90.0°, also with one molecule occupying the crystallographic asymmetric unit | uncultured bacterium |
Protein Variants | Comment | Organism |
---|---|---|
N14H | thermostabilizing mutation, catalytic activity is broadly similar to the wild-type | uncultured bacterium |
N30V | thermostabilizing mutation | uncultured bacterium |
Q10S | thermostabilizing mutation | uncultured bacterium |
Q34C | thermostabilizing mutation | uncultured bacterium |
Q34H | thermostabilizing mutation | uncultured bacterium |
Q34L | thermostabilizing mutation, catalytic activity is broadly similar to the wild-type | uncultured bacterium |
S194H | thermostabilizing mutation | uncultured bacterium |
S25E | thermostabilizing mutation | uncultured bacterium |
S35E | thermostabilizing mutation, catalytic activity is broadly similar to the wild-type | uncultured bacterium |
S71T | thermostabilizing mutation, catalytic activity is broadly similar to the wild-type | uncultured bacterium |
S9P | locks the conformation of a surface loop, catalytic activity is broadly similar to the wild-type | uncultured bacterium |
S9P | thermostabilizing mutation | uncultured bacterium |
S9P/T13F/N14H/Y18F/Q34L/S35E/S71T | hyperthermostable mutant Xyn11TS, retains full activity after incubation at 90°C for 60 min, catalytic activity is broadly similar to the wild-type | uncultured bacterium |
T13F | increases hydrophobic interactions | uncultured bacterium |
T13F | thermostabilizing mutation, catalytic activity is broadly similar to the wild-type | uncultured bacterium |
T13Y | thermostabilizing mutation | uncultured bacterium |
T4L | thermostabilizing mutation | uncultured bacterium |
Y18F | thermostabilizing mutation, catalytic activity is broadly similar to the wild-type | uncultured bacterium |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | B2LWN3 | - |
- |
Purification (Comment) | Organism |
---|---|
mutants and full-length protein Xyn11 purified by cation exchange chromatography and gel filtration, to more than 95% electrophoretic purity | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl beta-D-xylotrioside + H2O | - |
uncultured bacterium | ? | - |
? | |
oat-spelt xylan + H2O | - |
uncultured bacterium | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
GH11 xylanase | - |
uncultured bacterium |
xylanase | - |
uncultured bacterium |
Xyn11 | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
native enzyme | uncultured bacterium |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
Xyn11 in its apo form, is not subject to thermal inactivation up to 60°C | uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.317 | - |
oat-spelt xylan | hyperthermostable mutant Xyn11TS | uncultured bacterium | |
2.583 | - |
oat-spelt xylan | wild-type | uncultured bacterium | |
2.583 | - |
oat-spelt xylan | mutant S71T | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | 8 | recombinant form of full-length Xyn11, pH optimum of 8.0 using oat-spelled xylan and 7.2 when 4-nitrophenyl-beta-D-xylotrioside is the substrate | uncultured bacterium |