Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | stimulates activity with increasing amounts from 5 mM (16%) to 20 mM (31%) | Bacillus licheniformis | |
Co2+ | stimulates activity from 5 mM (16%) to 10 mM (26%) | Bacillus licheniformis | |
Cu2+ | stimulates activity at 5 mM by 20% | Bacillus licheniformis | |
Li+ | stimulates activity with increasing amounts from 5 mM (25%) to 20 mM (38%) | Bacillus licheniformis | |
Mg2+ | stimulates activity with increasing amounts from 5 mM (14%) to 20 mM (57%) | Bacillus licheniformis | |
Mn2+ | stimulates activity from 5 mM (27%) to 10 mM (40%) | Bacillus licheniformis | |
additional information | Fe2+ has little effect on enzyme activity from 5 mM to 10 mM | Bacillus licheniformis | |
Zn2+ | stimulates activity at 5 mM by 25% | Bacillus licheniformis |
Application | Comment | Organism |
---|---|---|
industry | the MS514-Xyn11 enzyme can be useful as an additive to mixtures designed to hydrolyze biomass as well as in applications for which it is desirable to maintain cellulose structure | Bacillus licheniformis |
Cloned (Comment) | Organism |
---|---|
expressed from vector pET29-MS14-X11 in Escherichia coli strain BL21(DE3)pLysE | Bacillus licheniformis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | inhibits activity from 10 mM (72%) to 20 mM (88%) | Bacillus licheniformis | |
Fe2+ | inhibits activity at 20 mM by 27% | Bacillus licheniformis | |
Mn2+ | inhibits activity at 20 mM by 25% | Bacillus licheniformis | |
Zn2+ | inhibits activity from 10 mM (7%) to 20 mM (21%) | Bacillus licheniformis |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
23400 | - |
sequence analysis | Bacillus licheniformis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus licheniformis | B5SYI8 | - |
- |
Bacillus licheniformis MS5-14 | B5SYI8 | - |
- |
Purification (Comment) | Organism |
---|---|
to apparent homogeneity by gel filtration | Bacillus licheniformis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | highest activity against birchwood xylan | Bacillus licheniformis | ? | - |
? | |
birchwood xylan + H2O | highest activity against birchwood xylan | Bacillus licheniformis MS5-14 | ? | - |
? | |
additional information | has negligible activity against insoluble and soluble cellulose substrates (avicel and carboxymethyl cellulose, respectively) and other glucose-based substrates (laminarin and lichenan) | Bacillus licheniformis | ? | - |
? | |
additional information | has negligible activity against insoluble and soluble cellulose substrates (avicel and carboxymethyl cellulose, respectively) and other glucose-based substrates (laminarin and lichenan) | Bacillus licheniformis MS5-14 | ? | - |
? | |
oat spelt xylan + H2O | - |
Bacillus licheniformis | ? | - |
? | |
oat spelt xylan + H2O | - |
Bacillus licheniformis MS5-14 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Bacillus licheniformis |
Xyn11 | - |
Bacillus licheniformis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 50 | - |
Bacillus licheniformis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
is stable at temperatures up to 50°C. At 55°C, more than half of the activity is lost, and no activity remains at 60°C | Bacillus licheniformis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
- |
Bacillus licheniformis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
5 | 7 | - |
Bacillus licheniformis |