Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-mercaptoethanol | 10 mM enhances the activities of XynAS27, XynAS27cd, and XynAS27cdl by about 1.5, 1.7, and 1.4fold, respectively | Streptomyces sp. S27 |
Application | Comment | Organism |
---|---|---|
industry | XynAS27 may be a compelling tool for the food industry because it generates xylobiose (85% w/w) as the main product of xylan hydrolysis | Streptomyces sp. S27 |
Cloned (Comment) | Organism |
---|---|
intact, CBM-truncated and CBM-linker-truncated versions cloned into vector pET-22b(+) and expressed in Escherichia coli BL21 (DE3) | Streptomyces sp. S27 |
Protein Variants | Comment | Organism |
---|---|---|
additional information | truncated versions xynAS27cd (CBM-linker-truncated version) and xynAS27cdl (CBM-truncated version) show less pH and thermal stability, and less affinity and hydrolytic activity to insoluble substrate than the intact one | Streptomyces sp. S27 |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM inhibits about 60% of the activity of XynAS27, and less than 20% of the activities of XynAS27cd and XynAS27cdl | Streptomyces sp. S27 | |
Co2+ | 10 mM partially inhibits the activity of XynAS27 | Streptomyces sp. S27 | |
Cu2+ | strongly inhibits | Streptomyces sp. S27 | |
Fe3+ | 10 mM partially inhibits the activity of XynAS27 | Streptomyces sp. S27 | |
Hg2+ | almost completely inhibits | Streptomyces sp. S27 | |
Ni2+ | 10 mM partially inhibits the activity of XynAS27 | Streptomyces sp. S27 | |
Pb2+ | 10 mM partially inhibits the activity of XynAS27 | Streptomyces sp. S27 | |
SDS | almost completely inhibits | Streptomyces sp. S27 | |
Zn2+ | 10 mM partially inhibits the activity of XynAS27 | Streptomyces sp. S27 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
33000 | - |
x * 47000, SDS-PAGE, XynAS27. x * 33000, SDS-PAGE, XynAS27cd. x * 34000, SDS-PAGE, XynAS27cdl | Streptomyces sp. S27 |
34000 | - |
x * 47000, SDS-PAGE, XynAS27. x * 33000, SDS-PAGE, XynAS27cd. x * 34000, SDS-PAGE, XynAS27cdl | Streptomyces sp. S27 |
47000 | - |
x * 47000, SDS-PAGE, XynAS27. x * 33000, SDS-PAGE, XynAS27cd. x * 34000, SDS-PAGE, XynAS27cdl | Streptomyces sp. S27 |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. S27 | C3RYK8 | - |
- |
Purification (Comment) | Organism |
---|---|
intact, CBM-truncated and CBM-linker-truncated versions of the mature proteins purified to electrophoretic homogeneity, by ion exchange chromatography or gel filtration | Streptomyces sp. S27 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
790.7 | - |
XynAS27 | Streptomyces sp. S27 |
1251 | - |
XynAS27cdl | Streptomyces sp. S27 |
1279 | - |
XynAS27cd | Streptomyces sp. S27 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
birchwood xylan + H2O | with soluble xylan after 12 h of incubation, about 15% (w/w) of the total reaction products is xylose, and 85% is xylobiose | Streptomyces sp. S27 | D-xylose + xylobiose + ? | - |
? | |
additional information | carbohydrate-binding module (CBM) of XynAS27 plays a key role in the hydrolysis of insoluble substrate, and the CBM and linker region are also important for the enzyme stability, and the linker region contributes more | Streptomyces sp. S27 | ? | - |
? | |
oat spelt xylan + H2O | with soluble xylan after 12 h of incubation, about 15% (w/w) of the total reaction products is xylose, and 85% is xylobiose. Comparing with the soluble xylan, the activities of XynAS27, XynAS27cd, and XynAS27cdl on insoluble xylan are lower, about 56%, 23% and 24% of the activities on soluble substrate, respectively | Streptomyces sp. S27 | D-xylose + xylobiose + ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 47000, SDS-PAGE, XynAS27. x * 33000, SDS-PAGE, XynAS27cd. x * 34000, SDS-PAGE, XynAS27cdl | Streptomyces sp. S27 |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Streptomyces sp. S27 |
XynAS27 | - |
Streptomyces sp. S27 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.2 | 12 | - |
Streptomyces sp. S27 |