Literature summary for 3.2.1.8 extracted from

Li, N.; Shi, P.; Yang, P.; Wang, Y.; Luo, H.; Bai, Y.; Zhou, Z.; Yao, B.
A xylanase with high pH stability from Streptomyces sp. S27 and its carbohydrate-binding module with/without linker-region-truncated versions (2009), Appl. Microbiol. Biotechnol., 83, 99-107.

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Activating Compound

Activating Compound	Comment	Organism	Structure
beta-mercaptoethanol	10 mM enhances the activities of XynAS27, XynAS27cd, and XynAS27cdl by about 1.5, 1.7, and 1.4fold, respectively	Streptomyces sp. S27

Application

Application	Comment	Organism
industry	XynAS27 may be a compelling tool for the food industry because it generates xylobiose (85% w/w) as the main product of xylan hydrolysis	Streptomyces sp. S27

Cloned(Commentary)

Cloned (Comment)	Organism
intact, CBM-truncated and CBM-linker-truncated versions cloned into vector pET-22b(+) and expressed in Escherichia coli BL21 (DE3)	Streptomyces sp. S27

Protein Variants

Protein Variants	Comment	Organism
additional information	truncated versions xynAS27cd (CBM-linker-truncated version) and xynAS27cdl (CBM-truncated version) show less pH and thermal stability, and less affinity and hydrolytic activity to insoluble substrate than the intact one	Streptomyces sp. S27

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	1 mM inhibits about 60% of the activity of XynAS27, and less than 20% of the activities of XynAS27cd and XynAS27cdl	Streptomyces sp. S27
Co2+	10 mM partially inhibits the activity of XynAS27	Streptomyces sp. S27
Cu2+	strongly inhibits	Streptomyces sp. S27
Fe3+	10 mM partially inhibits the activity of XynAS27	Streptomyces sp. S27
Hg2+	almost completely inhibits	Streptomyces sp. S27
Ni2+	10 mM partially inhibits the activity of XynAS27	Streptomyces sp. S27
Pb2+	10 mM partially inhibits the activity of XynAS27	Streptomyces sp. S27
SDS	almost completely inhibits	Streptomyces sp. S27
Zn2+	10 mM partially inhibits the activity of XynAS27	Streptomyces sp. S27

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
33000	-	x * 47000, SDS-PAGE, XynAS27. x * 33000, SDS-PAGE, XynAS27cd. x * 34000, SDS-PAGE, XynAS27cdl	Streptomyces sp. S27
34000	-	x * 47000, SDS-PAGE, XynAS27. x * 33000, SDS-PAGE, XynAS27cd. x * 34000, SDS-PAGE, XynAS27cdl	Streptomyces sp. S27
47000	-	x * 47000, SDS-PAGE, XynAS27. x * 33000, SDS-PAGE, XynAS27cd. x * 34000, SDS-PAGE, XynAS27cdl	Streptomyces sp. S27

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp. S27	C3RYK8	-	-

Purification (Commentary)

Purification (Comment)	Organism
intact, CBM-truncated and CBM-linker-truncated versions of the mature proteins purified to electrophoretic homogeneity, by ion exchange chromatography or gel filtration	Streptomyces sp. S27

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
790.7	-	XynAS27	Streptomyces sp. S27
1251	-	XynAS27cdl	Streptomyces sp. S27
1279	-	XynAS27cd	Streptomyces sp. S27

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
birchwood xylan + H2O	with soluble xylan after 12 h of incubation, about 15% (w/w) of the total reaction products is xylose, and 85% is xylobiose	Streptomyces sp. S27	D-xylose + xylobiose + ?	-	?
additional information	carbohydrate-binding module (CBM) of XynAS27 plays a key role in the hydrolysis of insoluble substrate, and the CBM and linker region are also important for the enzyme stability, and the linker region contributes more	Streptomyces sp. S27	?	-	?
oat spelt xylan + H2O	with soluble xylan after 12 h of incubation, about 15% (w/w) of the total reaction products is xylose, and 85% is xylobiose. Comparing with the soluble xylan, the activities of XynAS27, XynAS27cd, and XynAS27cdl on insoluble xylan are lower, about 56%, 23% and 24% of the activities on soluble substrate, respectively	Streptomyces sp. S27	D-xylose + xylobiose + ?	-	?

Subunits

Subunits	Comment	Organism
?	x * 47000, SDS-PAGE, XynAS27. x * 33000, SDS-PAGE, XynAS27cd. x * 34000, SDS-PAGE, XynAS27cdl	Streptomyces sp. S27

Synonyms

Synonyms	Comment	Organism
xylanase	-	Streptomyces sp. S27
XynAS27	-	Streptomyces sp. S27

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
2.2	12	-	Streptomyces sp. S27

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Release 2023.1 (January 2023)