Literature summary for 3.2.1.8 extracted from

Li, N.; Shi, P.; Yang, P.; Wang, Y.; Luo, H.; Bai, Y.; Zhou, Z.; Yao, B.
Cloning, expression, and characterization of a new Streptomyces sp. S27 xylanase for which xylobiose is the main hydrolysis product (2008), Appl. Biochem. Biotechnol., 159, 521-531.

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Activating Compound

Activating Compound	Comment	Organism	Structure
beta-mercaptoethanol	enhances the activity about 1.3- and 1.6fold at the concentrations of 1 and 10 mM, respectively	Streptomyces sp. S27
additional information	expression level of XynBS27 increases with induction time	Streptomyces sp. S27

Application

Application	Comment	Organism
industry	XynBS27 has several advantageous properties such as high specific activity, good activity over broad pH range, excellent pH stability, and being suitable for xylobiose production. These significant characteristics suggest that XynBS27 may be a good candidate in industrial application	Streptomyces sp. S27

Cloned(Commentary)

Cloned (Comment)	Organism
into the pPIC9 vector and overexpressed in Pichia pastoris GS115	Streptomyces sp. S27

Inhibitors

Inhibitors	Comment	Organism	Structure
Ag+	strongly inhibits by 69.1% even at the concentration of 1 mM	Streptomyces sp. S27
Ca2+	10 mM reduces the enzyme activity by 9.7%	Streptomyces sp. S27
Co2+	10 mM reduces the enzyme activity by 26.1%	Streptomyces sp. S27
Cu2+	10 mM reduces the enzyme activity by 49.6%	Streptomyces sp. S27
Fe2+	10 mM reduces the enzyme activity by 31.0%	Streptomyces sp. S27
Fe3+	10 mM reduces the enzyme activity by 11.3%	Streptomyces sp. S27
Hg2+	1 mM completely inhibits	Streptomyces sp. S27
K+	10 mM reduces the enzyme activity by 11.1%	Streptomyces sp. S27
Li+	10 mM reduces the enzyme activity by 16.1%	Streptomyces sp. S27
additional information	EDTA, Ni2+, Zn2+ and Mg2+ have no effect on activity	Streptomyces sp. S27
Na+	10 mM reduces the enzyme activity by 9.8%	Streptomyces sp. S27
Pb2+	10 mM reduces the enzyme activity by 32.9%	Streptomyces sp. S27
SDS	10 mM reduces the enzyme activity by 59.2%	Streptomyces sp. S27

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Cr3+	10 mM enhances the enzyme activity by 16.3%	Streptomyces sp. S27

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
21000	-	sequence analysis	Streptomyces sp. S27

Organism

Organism	UniProt	Comment	Textmining
Streptomyces sp. S27	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant XynBS27 purified to electrophoretic homogeneity by anion exchange chromatography	Streptomyces sp. S27

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3272	-	purified enzyme	Streptomyces sp. S27

Storage Stability

Storage Stability	Organism
4°C	Streptomyces sp. S27

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
birchwood xylan + H2O	main hydrolysis product of xylan is xylobiose (more than 75%)	Streptomyces sp. S27	D-xylose + xylobiose + xylotriose + ?	-	?
oat spelt xylan + H2O	main hydrolysis product of xylan is xylobiose (more than 75%)	Streptomyces sp. S27	xylobiose + D-xylose + xylotriose + ?	-	?

Synonyms

Synonyms	Comment	Organism
xylanase	-	Streptomyces sp. S27
XynBS27	-	Streptomyces sp. S27

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
65	-	-	Streptomyces sp. S27

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
1737	-	birchwood xylan	-	Streptomyces sp. S27
3475	-	oat spelt xylan	-	Streptomyces sp. S27

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Streptomyces sp. S27

pH Stability

pH Stability	pH Stability Maximum	Comment	Organism
4	12	XynBS27 shows good pH stability and retains more than 80% of the maximum activity after incubation at 37°C for 1 h. Between 5-8 retains over 65% of its activity	Streptomyces sp. S27

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Release 2023.1 (January 2023)