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Literature summary for 3.2.1.8 extracted from
- Purification, crystallization and crystallographic analysis of Clostridium thermocellum endo-1,4-beta-D-xylanase 10B in complex with xylohexaose (2008), Acta Crystallogr. Sect. F, 64, 715-718.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into pGEM T-easy and pET-21a vector, expressed in Escherichia coli XL10-Gold and BL21 (DE3) | Acetivibrio thermocellus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| N-terminal moiety of Xyn10B (residues 32-551), comprising a carbohydrate-binding module (CBM22-1) and the GH10 E337A mutant crystallized in complex with xylohexaose, by the hanging-drop vapour-phase diffusion method, to beyond 2.0 A resolution. Crystals belong to space group P3221 and contain a dimer in the asymmetric unit | Acetivibrio thermocellus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E337A | capture of the substrate xylohexaose in the inactivated site | Acetivibrio thermocellus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | P51584 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| mutant purified by immobilized metal-ion affinity chromatography and gel-filtration | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylohexaose + H2O | - |
Acetivibrio thermocellus | ? | - |
? |  |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| endo-1,4-beta-D-xylanase | - |
Acetivibrio thermocellus |
| xylanase | - |
Acetivibrio thermocellus |
| Xyn10B | - |
Acetivibrio thermocellus |
| XynY | - |
Acetivibrio thermocellus |
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