Cloned (Comment) | Organism |
---|---|
into pGEM T-easy and pET-21a vector, expressed in Escherichia coli XL10-Gold and BL21 (DE3) | Acetivibrio thermocellus |
Crystallization (Comment) | Organism |
---|---|
N-terminal moiety of Xyn10B (residues 32-551), comprising a carbohydrate-binding module (CBM22-1) and the GH10 E337A mutant crystallized in complex with xylohexaose, by the hanging-drop vapour-phase diffusion method, to beyond 2.0 A resolution. Crystals belong to space group P3221 and contain a dimer in the asymmetric unit | Acetivibrio thermocellus |
Protein Variants | Comment | Organism |
---|---|---|
E337A | capture of the substrate xylohexaose in the inactivated site | Acetivibrio thermocellus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acetivibrio thermocellus | P51584 | - |
- |
Purification (Comment) | Organism |
---|---|
mutant purified by immobilized metal-ion affinity chromatography and gel-filtration | Acetivibrio thermocellus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylohexaose + H2O | - |
Acetivibrio thermocellus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endo-1,4-beta-D-xylanase | - |
Acetivibrio thermocellus |
xylanase | - |
Acetivibrio thermocellus |
Xyn10B | - |
Acetivibrio thermocellus |
XynY | - |
Acetivibrio thermocellus |