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Literature summary for 3.2.1.8 extracted from
- Expression of recombinant Bacillus licheniformis xylanase A in Pichia pastoris and xylooligosaccharides released from xylans by it (2008), Protein Expr. Purif., 57, 101-107.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expressed in Pichia pastoris strain GS115 | Bacillus licheniformis |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 20000 | - |
SDS-PAGE | Bacillus licheniformis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bacillus licheniformis | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| ammonium sulfate precipitation and Sephadex G-100 gel filtration | Bacillus licheniformis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | xylan from birchwood and wheat bran insoluble xylan | Bacillus licheniformis | additional information | reaction products are xylose to xylopentaose with xylotriose as the major product | ? |  |
| xylobiose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| xylohexaose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| xylopentaose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| xylotetraose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
| xylotriose + H2O | - |
Bacillus licheniformis | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BlxA | - |
Bacillus licheniformis |
| xylanase A | - |
Bacillus licheniformis |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
- |
Bacillus licheniformis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | - |
the recombinant enzyme is stable below 70°C, enzyme activity is rapidly lost above 70°C, the residual activity of the recombinant BlxA is 76% at 70°C and pH 6.0 after 2 min | Bacillus licheniformis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Bacillus licheniformis |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Bacillus licheniformis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 9 | over 80% of recombinant BlxA activity is retained after treatment of the enzyme by preincubation over a pH range of 5.0-9.0 for 1 h at 25°C | Bacillus licheniformis |
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Release 2023.1 (January 2023)
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