Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21 (DE3) cells | Bacillus subtilis |
Protein Variants | Comment | Organism |
---|---|---|
F48C | mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme | Bacillus subtilis |
T44C | mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme | Bacillus subtilis |
T44Y | mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme | Bacillus subtilis |
T87D | mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme | Bacillus subtilis |
Y94C | mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme | Bacillus subtilis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus subtilis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA spin column chromatography | Bacillus subtilis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
wheat arabinoxylan + H2O | - |
Bacillus subtilis | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
endoxylanase | - |
Bacillus subtilis |
XynA | - |
Bacillus subtilis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
the wild type enzyme completely loses activity after preincubation at 60°C | Bacillus subtilis |