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Literature summary for 3.2.1.8 extracted from

  • Belien, T.; Verjans, P.; Courtin, C.M.; Delcour, J.A.
    Phage display based identification of novel stabilizing mutations in glycosyl hydrolase family 11 B. subtilis endoxylanase XynA (2008), Biochem. Biophys. Res. Commun., 368, 74-80.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21 (DE3) cells Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
F48C mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme Bacillus subtilis
T44C mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme Bacillus subtilis
T44Y mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme Bacillus subtilis
T87D mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme Bacillus subtilis
Y94C mutation increases the half-inactivation temperature by 2-3°C over that of the wild type enzyme Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni-NTA spin column chromatography Bacillus subtilis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
wheat arabinoxylan + H2O
-
Bacillus subtilis ?
-
?

Synonyms

Synonyms Comment Organism
endoxylanase
-
Bacillus subtilis
XynA
-
Bacillus subtilis

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
60
-
the wild type enzyme completely loses activity after preincubation at 60°C Bacillus subtilis