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Literature summary for 3.2.1.8 extracted from
- Purification and characterization of xylanases from Aspergillus giganteus (2004), Folia Microbiol. (Praha), 49, 13-18.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 2,4-dithiothreitol | stimulation, both xylanase I and xylanase II | Aspergillus giganteus |  |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | strong, both xylanase I and xylanase II | Aspergillus giganteus | |
| Hg2+ | strong, both xylanase I and xylanase II | Aspergillus giganteus | |
| sodium dodecylsulfate | strong, both xylanase I and xylanase II | Aspergillus giganteus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 21000 | - |
x * 21000, xylanase I, x * 24000, xylanase II, SDS-PAGE | Aspergillus giganteus |
| 24000 | - |
x * 21000, xylanase I, x * 24000, xylanase II, SDS-PAGE | Aspergillus giganteus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus giganteus | - |
isoforms xylanase I, xylanase II, growth in xylan | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| glycoprotein | xylanase I, about 56% carbohydtate content, xylanase II, about 62% carbohydrate | Aspergillus giganteus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| isoforms xylanase I, xylanase II | Aspergillus giganteus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| xylan + H2O | oatspelt xylan is preferred substrate | Aspergillus giganteus | xylotriose + xylotetraose + larger xylooligosaccharides | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 21000, xylanase I, x * 24000, xylanase II, SDS-PAGE | Aspergillus giganteus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
both xylanase I and xylanase II | Aspergillus giganteus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
xylanase I, half-life 22.5 min, xylanase II, half-life 17.5 min | Aspergillus giganteus |
| 60 | - |
xylanase I, half-life 1.5 min, xylanase II, half-life 1.5 min | Aspergillus giganteus |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 407 | - |
xylan | xylanase II, birchwood xylan, pH 6.0, 50°C | Aspergillus giganteus | |
| 468 | - |
xylan | xylanase II, oatspelt xylan, pH 6.0, 50°C | Aspergillus giganteus | |
| 1031 | - |
xylan | xylanase I, birchwood xylan, pH 6.0, 50°C | Aspergillus giganteus | |
| 1345 | - |
xylan | xylanase I, oatspelt xylan, pH 6.0, 50°C | Aspergillus giganteus | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 6.5 | xylanase I | Aspergillus giganteus |
| 6 | - |
xylanase II | Aspergillus giganteus |
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