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Literature summary for 3.2.1.8 extracted from
- Cloning, functional expression and characterization of three Phanerochaete chrysosporium endo-1,4-beta-xylanases (2004), Curr. Genet., 46, 166-175.
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 2 mM, 20% residual activity for isoforms xynA, xynC, 67% residual activity for isoform xynB | Phanerodontia chrysosporium |  |
| Pb2+ | 2 mM, 70% residual activity for isoforms xynA, xynC, 80% residual activity for isoform xynB | Phanerodontia chrysosporium | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 28600 | - |
x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE | Phanerodontia chrysosporium |
| 32000 | - |
x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE | Phanerodontia chrysosporium |
| 40300 | - |
x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE | Phanerodontia chrysosporium |
| 41600 | - |
x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE | Phanerodontia chrysosporium |
| 50000 | - |
x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE | Phanerodontia chrysosporium |
| 52000 | - |
x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE | Phanerodontia chrysosporium |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Phanerodontia chrysosporium | - |
isoforms xynA, xynC, family 10 glycoside hydrolases, xynB, family 11 glycoside hydrolase | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| additional information | sequences contain several potential O-glycosylation sites, sequence of xynB contains addtitionally a potential N-glycosylation site | Phanerodontia chrysosporium |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| after expression in Aspergillus niger | Phanerodontia chrysosporium |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| p-nitrophenyl beta-D-cellobioside + H2O | weak substrate for isoforms xynA, xynC, no substrate for xynB | Phanerodontia chrysosporium | ? | - |
? | |
| p-nitrophenyl beta-D-xylopyranoside + H2O | weak substrate for isoforms xynA, xynC, no substrate for xynB | Phanerodontia chrysosporium | p-nitrophenol + beta-D-xylopyranoside | - |
? | |
| xylan + H2O | from birchwood, beechwood or oatspelt. Oatspelt xylan is slightly preferred | Phanerodontia chrysosporium | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 41600, isoform xynA, x * 28600, isoform xynB, x * 40300, isoform xynC, calculated, x * 52000, isoform xynA, x * 50000, isoform xynB, x * 32000, isoform xynC, SDS-PAGE | Phanerodontia chrysosporium |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
isoform xynB | Phanerodontia chrysosporium |
| 70 | - |
isoforms xynA, xynC | Phanerodontia chrysosporium |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
isoform xynB, stable up to | Phanerodontia chrysosporium |
| 60 | - |
isoforms xynA, xynC, stable for 30 min | Phanerodontia chrysosporium |
| 70 | - |
isoforms xynA, xynB, xynC, complete inactivation in 30 min | Phanerodontia chrysosporium |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
isoforms xynA, xynB, xynC | Phanerodontia chrysosporium |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 8 | isoforms xynA, xynB, stable for at least 2 h | Phanerodontia chrysosporium |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Phanerodontia chrysosporium | isoform xynC, calculated | - |
5.5 |
| Phanerodontia chrysosporium | isoform xynA, calculated | - |
5.8 |
| Phanerodontia chrysosporium | isoform xynB, calculated | - |
6 |
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