K58R |
site-directed mutagenesis, slight increase of thermostability at 55°C from half-life 5 min, wild-type, to 10-20 min, increased pH-stability compared to the wild-type enzyme |
Trichoderma reesei |
K58R/A160R |
site-directed mutagenesis, no alteration of thermal or pH-stability |
Trichoderma reesei |
K58R/A160R/N97R |
site-directed mutagenesis, no alteration of thermal or pH-stability |
Trichoderma reesei |
K58R/A160R/N97R/N67R |
site-directed mutagenesis, no alteration of thermal or pH-stability |
Trichoderma reesei |
K58R/A160R/N97R/N67R/T26R |
site-directed mutagenesis, no alteration of thermal or pH-stability |
Trichoderma reesei |
K58R/A160R/N97R/N67R/T26R/A132R |
site-directed mutagenesis, no alteration of thermal or pH-stability |
Trichoderma reesei |
additional information |
construction of several mutant with increased number of arginines on the protein surface showing unaltered thermal stability but narrowed pH optimum, mutation of arginines to Ser/Thr causes a pH profile shift |
Trichoderma reesei |
S186R |
site-directed mutagenesis, reduced thermal stability at 50°C in absence of substrate compared to the wild-type enzyme |
Trichoderma reesei |
S186R/N67R |
site-directed mutagenesis, reduced thermal stability at 50°C in absence of substrate compared to the wild-type enzyme |
Trichoderma reesei |
S186R/N67R/T26R |
site-directed mutagenesis, reduced thermal stability at 50°C in absence of substrate, reduced stability at 60°C and unaltered at 65°C in presence of substrate, compared to the wild-type enzyme |
Trichoderma reesei |
S186R/N67R/T26R/Q34R |
site-directed mutagenesis, highly reduced thermal stability at 50°C in absence of substrate, unaltered stability at 60°C and slightly increased at 65°C in presence of substrate, compared to the wild-type enzyme |
Trichoderma reesei |
S186R/N67R/T26R/Q34R/N69R |
site-directed mutagenesis, reduced thermal stability at 50°C in absence of substrate, increased stability at 60°C and 65°C in presence of substrate, compared to the wild-type enzyme |
Trichoderma reesei |
S186R/N67R/T26R/Q34R/S40R |
site-directed mutagenesis, highly reduced thermal stability at 50°C in absence of substrate, highly increased stability at 60°C and increased 65°C in presence of substrate, compared to the wild-type enzyme |
Trichoderma reesei |