Application | Comment | Organism |
---|---|---|
paper production | enzyme has biodelignification potential and can be used in bleaching of kraft pulps, method development, overview | Streptomyces sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Streptomyces sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | secretion of enzymes | Streptomyces sp. | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24500 | - |
x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE | Streptomyces sp. |
37500 | - |
x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE | Streptomyces sp. |
38000 | - |
x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE | Streptomyces sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | Streptomyces sp. | delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 | ? | - |
? | |
xylan + H2O | Streptomyces sp. S38 | delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces sp. | - |
enzymes Xyl1, Xyl2, and Xyl3 | - |
Streptomyces sp. S38 | - |
enzymes Xyl1, Xyl2, and Xyl3 | - |
Purification (Comment) | Organism |
---|---|
3 enzymes to homogeneity from culture supernatant | Streptomyces sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | highest enzyme production rate from culture on minimal medium at pH 7.0 and 55°C | Streptomyces sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Streptomyces sp. |
5700 | - |
purified Xyl1, substrate birchwood xylan, at 50°C | Streptomyces sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-nitrophenyl arabinofuranoside + H2O | Xyl2 and Xyl3 show arabinofuranosidase activity | Streptomyces sp. | 4-nitrophenol + arabinofuranose | - |
? | |
4-nitrophenyl arabinofuranoside + H2O | Xyl2 and Xyl3 show arabinofuranosidase activity | Streptomyces sp. S38 | 4-nitrophenol + arabinofuranose | - |
? | |
4-nitrophenyl cellobioside + H2O | Xyl2 and Xyl3 show cellulase activity | Streptomyces sp. | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl cellobioside + H2O | Xyl2 and Xyl3 show cellulase activity | Streptomyces sp. S38 | 4-nitrophenol + cellobiose | - |
? | |
4-nitrophenyl xylopyranoside + H2O | Xyl2 and Xyl3 show xylosidase activity | Streptomyces sp. | 4-nitrophenol + xylopyranose | - |
? | |
additional information | no activity on CM-cellulose | Streptomyces sp. | ? | - |
? | |
additional information | no activity on CM-cellulose | Streptomyces sp. S38 | ? | - |
? | |
xylan + H2O | delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 | Streptomyces sp. | ? | - |
? | |
xylan + H2O | from birchwood or oat spelt | Streptomyces sp. | ? | - |
? | |
xylan + H2O | delignification of hardwood and softwood, the enzymes are differing in their bleaching potential, Xyl1 is more effective tahn Xyl2 and Xyl3 | Streptomyces sp. S38 | ? | - |
? | |
xylan + H2O | from birchwood or oat spelt | Streptomyces sp. S38 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 24500, Xyl1, SDS-PAGE, x * 37500, Xyl2, SDS-PAGE, x * 38000, Xyl3, SDS-PAGE | Streptomyces sp. |
Synonyms | Comment | Organism |
---|---|---|
endo-beta-1,4-xylanase | - |
Streptomyces sp. |
More | Xyl1 belongs to family 11, while Xyl2 and Xyl3 belong to family 10 of glycohydrolases | Streptomyces sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Streptomyces sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
50 | - |
Xyl1-Xyl3 are stable for 16 h | Streptomyces sp. |
60 | - |
half-lives: Xyl1 10 min, Xyl2 30 min, Xyl3 70 min | Streptomyces sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | 7.5 | Xyl2 | Streptomyces sp. |
6 | 6.5 | Xyl1 | Streptomyces sp. |
6 | 8 | Xyl3 | Streptomyces sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
3 | 10 | Xyl1-Xyl3, no loss of activity in 7 days at 20°C | Streptomyces sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Streptomyces sp. | Xyl2, isoelectric focusing | - |
4.9 |
Streptomyces sp. | Xyl3, isoelectric focusing | - |
4.9 |
Streptomyces sp. | Xyl1, isoelectric focusing | - |
5.5 |