Cloned (Comment) | Organism |
---|---|
gene XynA, overexpression in Pichia pastoris, method optimization, functional expression under control of the AOX1 promotor | Thermomyces lanuginosus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermomyces lanuginosus | O43097 | gene XynA | - |
Thermomyces lanuginosus IOC-4145 | O43097 | gene XynA | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Pichia pastoris, and native enzyme, by gel filtration | Thermomyces lanuginosus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
xylan + H2O | - |
Thermomyces lanuginosus | ? | - |
? | |
xylan + H2O | - |
Thermomyces lanuginosus IOC-4145 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21900-22300, recombinant enzyme, SDS-PAGE | Thermomyces lanuginosus |
Synonyms | Comment | Organism |
---|---|---|
xylanase | - |
Thermomyces lanuginosus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
75 | - |
native and recombinant enzyme | Thermomyces lanuginosus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
80 min, 60% remaining activity, native and recombinant enzyme | Thermomyces lanuginosus |
80 | - |
40 min, 60% remaining activity, native and recombinant enzyme | Thermomyces lanuginosus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
assay at | Thermomyces lanuginosus |