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Literature summary for 3.2.1.8 extracted from
- Production , purification and characterization of xylanase from hyperxylanolytic mutant of Aspergillus ochraceus (1990), Biotechnol. Bioeng., 35, 244-251.
General Stability
| General Stability | Organism |
|---|---|
| K+ stabilizes against thermal inactivation | Aspergillus ochraceus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | CaCl2 | Aspergillus ochraceus |  |
| Cd2+ | CdCl2 | Aspergillus ochraceus | |
| Co2+ | CoCl2 | Aspergillus ochraceus | |
| Cu2+ | CuSO4 | Aspergillus ochraceus | |
| DTNB | - |
Aspergillus ochraceus | |
| Fe2+ | FeCl2, FeSO4 | Aspergillus ochraceus | |
| Hg2+ | HgCl2 | Aspergillus ochraceus | |
| MgCl2 | - |
Aspergillus ochraceus | |
| Mn2+ | MnCl2 | Aspergillus ochraceus | |
| NEM | - |
Aspergillus ochraceus | |
| Ni2+ | NiCl2 | Aspergillus ochraceus | |
| PHMB | - |
Aspergillus ochraceus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Aspergillus ochraceus |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus ochraceus | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| K+ | slight stimulation | Aspergillus ochraceus | |
| Na+ | slight stimulation | Aspergillus ochraceus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 48000 | - |
1 * 48000, SDS-PAGE | Aspergillus ochraceus |
| 50000 | - |
gel filtration | Aspergillus ochraceus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-beta-D-xylan + H2O | Aspergillus ochraceus | enzyme is secreted at high levels during growth on commercial xylan and on agricultural wastes | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus ochraceus | - |
wild-type and mutant strain NG-13 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Aspergillus ochraceus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-beta-D-xylan + H2O | larchwood xylan | Aspergillus ochraceus | additional information | xylobiose + xylotriose are produced from larchwood xylan | ? | |
| 1,4-beta-D-xylan + H2O | enzyme is secreted at high levels during growth on commercial xylan and on agricultural wastes | Aspergillus ochraceus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 48000, SDS-PAGE | Aspergillus ochraceus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Aspergillus ochraceus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| additional information | - |
K+ stabilizes against thermal inactivation | Aspergillus ochraceus |
| 45 | - |
37.5% loss of activity after 90 min, stable up to 30 min | Aspergillus ochraceus |
| 50 | - |
87.5% loss of activity after 90 min | Aspergillus ochraceus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Aspergillus ochraceus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 10 | 45°C, 30 min, stable | Aspergillus ochraceus |
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