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Literature summary for 3.2.1.8 extracted from

  • Kitpreechavanich, V.; Hayashi, M.; Nagai, S.
    Purification and properties of endo-1,4-beta-xylanase from Humicola lanuginosa (1984), J. Ferment. Technol., 62, 415-420.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Co2+ 10 mM Thermomyces lanuginosus
Cu2+ 10 mM Thermomyces lanuginosus
Fe3+ 10 mM Thermomyces lanuginosus
Hg2+ 10 mM Thermomyces lanuginosus
xylobiose competitive Thermomyces lanuginosus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Thermomyces lanuginosus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
gel filtration Thermomyces lanuginosus
21500
-
1 * 21500, SDS-PAGE Thermomyces lanuginosus

Organism

Organism UniProt Comment Textmining
Thermomyces lanuginosus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermomyces lanuginosus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Thermomyces lanuginosus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O
-
Thermomyces lanuginosus additional information xylooligosaccharides are observed at the beginning and after prolonged incubation, xylotriose and xylobiose are predominant, with a small amount of xylose ?
4-nitrophenyl beta-D-xylopyranoside + H2O no hydrolysis Thermomyces lanuginosus 4-nitrophenol + beta-D-xylopyranose
-
?
starch + H2O no activity Thermomyces lanuginosus ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 21500, SDS-PAGE Thermomyces lanuginosus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
-
Thermomyces lanuginosus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
30 min, stable up to Thermomyces lanuginosus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
-
Thermomyces lanuginosus

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 stable over the range Thermomyces lanuginosus