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Literature summary for 3.2.1.8 extracted from
- Purification and characterization of a thermostable xylanase from a thermophilic fungus Thermoascus aurantiacus (1987), Can. J. Microbiol., 33, 689-692.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Hg2+ | 2 mM, complete loss of activity; 2 mM HgCl2, complete inactivation | Thermoascus aurantiacus |  |
| additional information | the xylanase was not affected by copper sulfate, zinc sulfate, calcium chloride, cobalt chloride, barium chloride, magnesium sulfate, and EDTA at concentrations of 2 mM | Thermoascus aurantiacus | |
| Pb(CH3COO)2 | 2 mM, activity is reduced by 25% | Thermoascus aurantiacus | |
| Pb2+ | 2 mM, 25% loss of activity | Thermoascus aurantiacus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | - |
Thermoascus aurantiacus | |
| additional information | - |
xylan | 0.17% w/v, 50°C | Thermoascus aurantiacus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 32000 | - |
- |
Thermoascus aurantiacus |
| 32000 | - |
1 * 32000, SDS-PAGE | Thermoascus aurantiacus |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Thermoascus aurantiacus | - |
- |
- |
| Thermoascus aurantiacus C436 | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Thermoascus aurantiacus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 42.4 | - |
- |
Thermoascus aurantiacus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 1,4-beta-D-xylan + H2O | - |
Thermoascus aurantiacus | additional information | - |
? | |
| 1,4-beta-D-xylan + H2O | - |
Thermoascus aurantiacus C436 | additional information | - |
? | |
| xylan + H2O | - |
Thermoascus aurantiacus | ? | - |
? | |
| xylan + H2O | - |
Thermoascus aurantiacus C436 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 32000, SDS-PAGE | Thermoascus aurantiacus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 80 | - |
- |
Thermoascus aurantiacus |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 70 | 85 | 70°C: about 70% of maximal activity, 85°C: about 10% of maximal activity | Thermoascus aurantiacus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 60 | - |
97 h, retains full activity | Thermoascus aurantiacus |
| 60 | - |
retains full activity for 97 h | Thermoascus aurantiacus |
| 70 | - |
24 h, retains full activity | Thermoascus aurantiacus |
| 70 | - |
retains full activity for 24 h | Thermoascus aurantiacus |
| 80 | - |
half-life: 54 min | Thermoascus aurantiacus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.1 | - |
- |
Thermoascus aurantiacus |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3.5 | 7.5 | 12% of maximal activity at pH 3.5 and at pH 7.5 | Thermoascus aurantiacus |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Thermoascus aurantiacus | - |
- |
7.1 |
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