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Literature summary for 3.2.1.8 extracted from

  • Akiba, T.; Horikoshi, K.
    Xylanases of alkalophilic thermophilic Bacillus (1988), Methods Enzymol., 160, 655-659.
No PubMed abstract available

Inhibitors

Inhibitors Comment Organism Structure
Ag+ 5 mM, enzyme component I and II of strain W1 and W2, partial inhibition Bacillus sp. (in: Bacteria)
Cu2+ 5 mM, enzyme component I and II of strain W1 and W2, partial inhibition Bacillus sp. (in: Bacteria)
EDTA 5 mM, enzyme component I and II of strain W1 and W2, partial inhibition Bacillus sp. (in: Bacteria)
Hg2+ 5 mM, enzyme component I and II of strain W1 and W2, completely inhibited Bacillus sp. (in: Bacteria)
mercuribenzoate 5 mM, 40% inhibition of enzyme component I of strain W1 and W2 Bacillus sp. (in: Bacteria)

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information
-
Bacillus sp. (in: Bacteria)

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
21500
-
x * 21500, enzyme component I from strain W1, SDS-PAGE Bacillus sp. (in: Bacteria)
22500
-
x * 22500, enzyme component II from strain W1, SDS-PAGE Bacillus sp. (in: Bacteria)
48500
-
enzyme component II from strain W1, gel filtration Bacillus sp. (in: Bacteria)
49500
-
1 * 49500, enzyme component II from strain W1, SDS-PAGE Bacillus sp. (in: Bacteria)
50000
-
1 * 50000, enzyme component II from strain W2, SDS-PAGE Bacillus sp. (in: Bacteria)
51000
-
enzyme component II from strain W2, gel filtration Bacillus sp. (in: Bacteria)

Organism

Organism UniProt Comment Textmining
Bacillus sp. (in: Bacteria)
-
strains W1 (JCM 2888) and W2 (JCM 2889)
-

Purification (Commentary)

Purification (Comment) Organism
-
Bacillus sp. (in: Bacteria)

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
-
Bacillus sp. (in: Bacteria)

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O
-
Bacillus sp. (in: Bacteria) additional information hydrolysis products formed by enzyme component I of strain W1 and W2 are xylobiose + xylotriose + xylotetraose + xylopentaose, xylose is not formed. Hydrolysis products formed by enzyme component I of strain W1 and W2 are xylose + xylobiose + xylotriose + xylopentaose, xylotetraose is not formed. Xylobiose is the predominant product ?
additional information transferase activity Bacillus sp. (in: Bacteria) ?
-
?
xylobiose + H2O no hydrolysis Bacillus sp. (in: Bacteria) ?
-
?
xylotriose + H2O
-
Bacillus sp. (in: Bacteria) xylobiose + xylose
-
?

Subunits

Subunits Comment Organism
? x * 21500, enzyme component I from strain W1, SDS-PAGE Bacillus sp. (in: Bacteria)
? x * 22500, enzyme component II from strain W1, SDS-PAGE Bacillus sp. (in: Bacteria)
monomer 1 * 50000, enzyme component II from strain W2, SDS-PAGE Bacillus sp. (in: Bacteria)
monomer 1 * 49500, enzyme component II from strain W1, SDS-PAGE Bacillus sp. (in: Bacteria)

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
65
-
enzyme component I from strain W1 and strain W2 Bacillus sp. (in: Bacteria)
70
-
enzyme component II from strain W1 and strain W2 Bacillus sp. (in: Bacteria)

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6
-
enzyme component I from strain W1 and strain W2 Bacillus sp. (in: Bacteria)
7 9 enzyme component II from strain W1 and strain W2 Bacillus sp. (in: Bacteria)

pH Stability

pH Stability pH Stability Maximum Comment Organism
4.5 10 1 h, 45°C, stable between pH 4.5 and 10.0, enzyme component I and II from strain W1 and W2, stable Bacillus sp. (in: Bacteria)