Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 5 mM, enzyme component I and II of strain W1 and W2, partial inhibition | Bacillus sp. (in: Bacteria) | |
Cu2+ | 5 mM, enzyme component I and II of strain W1 and W2, partial inhibition | Bacillus sp. (in: Bacteria) | |
EDTA | 5 mM, enzyme component I and II of strain W1 and W2, partial inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | 5 mM, enzyme component I and II of strain W1 and W2, completely inhibited | Bacillus sp. (in: Bacteria) | |
mercuribenzoate | 5 mM, 40% inhibition of enzyme component I of strain W1 and W2 | Bacillus sp. (in: Bacteria) |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | - |
Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
21500 | - |
x * 21500, enzyme component I from strain W1, SDS-PAGE | Bacillus sp. (in: Bacteria) |
22500 | - |
x * 22500, enzyme component II from strain W1, SDS-PAGE | Bacillus sp. (in: Bacteria) |
48500 | - |
enzyme component II from strain W1, gel filtration | Bacillus sp. (in: Bacteria) |
49500 | - |
1 * 49500, enzyme component II from strain W1, SDS-PAGE | Bacillus sp. (in: Bacteria) |
50000 | - |
1 * 50000, enzyme component II from strain W2, SDS-PAGE | Bacillus sp. (in: Bacteria) |
51000 | - |
enzyme component II from strain W2, gel filtration | Bacillus sp. (in: Bacteria) |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
strains W1 (JCM 2888) and W2 (JCM 2889) | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
1,4-beta-D-xylan + H2O | - |
Bacillus sp. (in: Bacteria) | additional information | hydrolysis products formed by enzyme component I of strain W1 and W2 are xylobiose + xylotriose + xylotetraose + xylopentaose, xylose is not formed. Hydrolysis products formed by enzyme component I of strain W1 and W2 are xylose + xylobiose + xylotriose + xylopentaose, xylotetraose is not formed. Xylobiose is the predominant product | ? | |
additional information | transferase activity | Bacillus sp. (in: Bacteria) | ? | - |
? | |
xylobiose + H2O | no hydrolysis | Bacillus sp. (in: Bacteria) | ? | - |
? | |
xylotriose + H2O | - |
Bacillus sp. (in: Bacteria) | xylobiose + xylose | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 21500, enzyme component I from strain W1, SDS-PAGE | Bacillus sp. (in: Bacteria) |
? | x * 22500, enzyme component II from strain W1, SDS-PAGE | Bacillus sp. (in: Bacteria) |
monomer | 1 * 50000, enzyme component II from strain W2, SDS-PAGE | Bacillus sp. (in: Bacteria) |
monomer | 1 * 49500, enzyme component II from strain W1, SDS-PAGE | Bacillus sp. (in: Bacteria) |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
65 | - |
enzyme component I from strain W1 and strain W2 | Bacillus sp. (in: Bacteria) |
70 | - |
enzyme component II from strain W1 and strain W2 | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6 | - |
enzyme component I from strain W1 and strain W2 | Bacillus sp. (in: Bacteria) |
7 | 9 | enzyme component II from strain W1 and strain W2 | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
4.5 | 10 | 1 h, 45°C, stable between pH 4.5 and 10.0, enzyme component I and II from strain W1 and W2, stable | Bacillus sp. (in: Bacteria) |