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Literature summary for 3.2.1.8 extracted from

  • Okada, H.; Shinmyo, A.
    Xylanase of Bacillus pumilus (1988), Methods Enzymol., 160, 632-637.
No PubMed abstract available
Search for more literature for 3.2.1.8

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Bacillus pumilus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
20000
-
 equilibrium sedimentation Bacillus pumilus
24000
-
 1 * 24000, SDS-PAGE Bacillus pumilus

Organism

Organism UniProt Comment Textmining
Bacillus pumilus
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no modification contains no carbohydrate Bacillus pumilus

Purification (Commentary)

Purification (Comment) Organism
-
 Bacillus pumilus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1780
-
-
 Bacillus pumilus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1,4-beta-D-xylan + H2O larchwood xylan Bacillus pumilus additional information xylose + xylobiose + xylotriose + higher oligomers ?
xylotriose trans-xylosidation activity Bacillus pumilus xylobiose + xylotetraose
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 24000, SDS-PAGE Bacillus pumilus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
45 50
-
 Bacillus pumilus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 half-life: 30 min Bacillus pumilus
60
-
 complete inactivation in 15 min Bacillus pumilus

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5
-
 stability optimum Bacillus pumilus