Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | Ca2+, Mg2+, Mn2+, Fe2+, Ni2+, and Zn2+ have either no effect or a slightly inhibitory effect | Reinekea sp. KIT-YO10 |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | Michaelis-Menten kinetics | Reinekea sp. KIT-YO10 |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
44300 | - |
x * 44300, SDS-PAGE | Reinekea sp. KIT-YO10 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
konjac glucomannan + H2O | Reinekea sp. KIT-YO10 | - |
? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Reinekea sp. KIT-YO10 | - |
isolated from the seashore of Kanazawa Port in Japan | - |
Purification (Comment) | Organism |
---|---|
native enzyme 29.4fold by anion exchange chromatography | Reinekea sp. KIT-YO10 |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
236 | - |
pH 8.0, 40°C, substrate konjac glucomannan | Reinekea sp. KIT-YO10 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
guar gum + H2O | 1.7% activity compared to konjac glucomannan | Reinekea sp. KIT-YO10 | ? | - |
? | |
ivory nut mannan + H2O | 46% activity compared to konjac glucomannan | Reinekea sp. KIT-YO10 | ? | - |
? | |
konjac glucomannan + H2O | - |
Reinekea sp. KIT-YO10 | ? | - |
? | |
konjac glucomannan + H2O | best substrate | Reinekea sp. KIT-YO10 | ? | - |
? | |
locust bean gum + H2O | 9.6% activity compared to konjac glucomannan | Reinekea sp. KIT-YO10 | ? | - |
? | |
additional information | no activity with tamarind xyloglucan, sodium arginate, carboxymethylcellulose, low-melting point agarose, gellan gum, xylan, carrageenan, and laminaran | Reinekea sp. KIT-YO10 | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 44300, SDS-PAGE | Reinekea sp. KIT-YO10 |
Synonyms | Comment | Organism |
---|---|---|
Beta-mannanase | - |
Reinekea sp. KIT-YO10 |
endo-1,4-beta-D-mannanase | - |
Reinekea sp. KIT-YO10 |
Rman | - |
Reinekea sp. KIT-YO10 |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
70 | - |
- |
Reinekea sp. KIT-YO10 |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | activity range, profile overview | Reinekea sp. KIT-YO10 |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | purified enzyme, pH 8.0, 20 min, stable at | Reinekea sp. KIT-YO10 |
70 | - |
purified enzyme, pH 8.0, 20 min, loss of 50% activity | Reinekea sp. KIT-YO10 |
90 | - |
purified enzyme, pH 8.0, 20 min, inactivation | Reinekea sp. KIT-YO10 |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
- |
Reinekea sp. KIT-YO10 |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
7 | 9 | over 80% of amximal activity | Reinekea sp. KIT-YO10 |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
8 | - |
purified enzyme, 30°C, 30 min, over 90% activity remaining | Reinekea sp. KIT-YO10 |