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Literature summary for 3.2.1.78 extracted from
- endo-beta-1,4-Mannanases from blue mussel, Mytilus edulis: purification, characterization, and mode of action (2002), J. Biotechnol., 92, 267-277.
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 39000 | - |
gel filtration, ManA and ManB | Mytilus edulis |
| 39216 | - |
1 * 39216, ManA, 1 * 39265, ManB, MALDI-TOF spectrometry | Mytilus edulis |
| 39265 | - |
1 * 39216, ManA, 1 * 39265, ManB, MALDI-TOF spectrometry | Mytilus edulis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Mytilus edulis | - |
isozymes ManA, ManB | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Mytilus edulis |
Renatured (Commentary)
| Renatured (Comment) | Organism |
|---|---|
| can be completely renatured after denaturation in 6 M guanidine hydrochloride | Mytilus edulis |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ivory nut mannan + H2O | - |
Mytilus edulis | ? | - |
? |  |
| locust bean gum + H2O | - |
Mytilus edulis | ? | - |
? | |
| additional information | no substrate: xylan, caroxymethyl cellulose, cellulose, mannan | Mytilus edulis | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 39216, ManA, 1 * 39265, ManB, MALDI-TOF spectrometry | Mytilus edulis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| ManA | - |
Mytilus edulis |
| ManB | - |
Mytilus edulis |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | 55 | - |
Mytilus edulis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
unstable above | Mytilus edulis |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
- |
Mytilus edulis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 4 | 9 | - |
Mytilus edulis |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Mytilus edulis | isoelectric focusing | - |
6.9 |
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