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Literature summary for 3.2.1.74 extracted from
- Degradation of cellulosome-produced cello-oligosaccharides by an extracellular non-cellulosomal beta-glucan glucohydrolase, BglA, from Clostridium cellulovorans (2006), Biochem. Biophys. Res. Commun., 349, 20-23.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Escherichia coli | Clostridium cellulovorans |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| D-glucose | at 20 mM | Clostridium cellulovorans |  |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 1.1 | - |
cellotetraose | - |
Clostridium cellulovorans | |
| 1.7 | - |
cellotriose | - |
Clostridium cellulovorans | |
| 2.9 | - |
cellobiose | - |
Clostridium cellulovorans | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 51570 | - |
calculated | Clostridium cellulovorans |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium cellulovorans | Q53EH2 | ATCC35296 | - |
| Clostridium cellulovorans ATCC 35296 | Q53EH2 | ATCC35296 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| Ni-nitrilotriacetatic acid agarose column chromatography | Clostridium cellulovorans |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 129 | - |
p-nitrophenyl-beta-D-fucopyranoside as substrate | Clostridium cellulovorans |
| 130 | - |
o-nitrophenyl-beta-D-galactopyranoside as substrate | Clostridium cellulovorans |
| 130 | - |
p-nitrophenyl-beta-D-cellobioside as substrate | Clostridium cellulovorans |
| 133 | - |
p-nitrophenyl-beta-D-galactopyranoside as substrate | Clostridium cellulovorans |
| 177 | - |
p-nitrophenyl-beta-D-glucopyranoside as substrate | Clostridium cellulovorans |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-nitrophenyl beta-D-glucopyranose + H2O | - |
Clostridium cellulovorans | 4-nitrophenol + D-glucose | - |
? | |
| cellobiose + H2O | - |
Clostridium cellulovorans | 2 D-glucose | - |
? | |
| cellobiose + H2O | - |
Clostridium cellulovorans ATCC 35296 | 2 D-glucose | - |
? | |
| cellooligomers + H2O | - |
Clostridium cellulovorans | D-glucose + ? | - |
? | |
| cellooligomers + H2O | - |
Clostridium cellulovorans ATCC 35296 | D-glucose + ? | - |
? | |
| cellotetraose + 3 H2O | - |
Clostridium cellulovorans | 4 D-glucose | - |
? | |
| cellotetraose + 3 H2O | - |
Clostridium cellulovorans ATCC 35296 | 4 D-glucose | - |
? | |
| cellotriose + H2O | - |
Clostridium cellulovorans | D-glucose + cellobiose | - |
? | |
| cellotriose + H2O | - |
Clostridium cellulovorans ATCC 35296 | D-glucose + cellobiose | - |
? | |
| o-nitrophenyl-beta-D-galactopyranose + H2O | - |
Clostridium cellulovorans | o-nitrophenol + beta-D-galactopyranose | - |
? | |
| o-nitrophenyl-beta-D-galactopyranose + H2O | - |
Clostridium cellulovorans ATCC 35296 | o-nitrophenol + beta-D-galactopyranose | - |
? | |
| p-nitrophenyl-beta-D-cellobiose + H2O | - |
Clostridium cellulovorans | p-nitrophenol + cellobioside | - |
? | |
| p-nitrophenyl-beta-D-fucopyranoside + H2O | - |
Clostridium cellulovorans | p-nitrophenol + fucopyranose | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| b-glucan glucohydrolase | extracellular non-cellulosomal beta-glucan glucohydrolase | Clostridium cellulovorans |
| BglA | - |
Clostridium cellulovorans |
| family-1 glycosyl hydrolase | - |
Clostridium cellulovorans |
| glucan 1,4-beta-glucosidase | - |
Clostridium cellulovorans |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 50 | - |
- |
Clostridium cellulovorans |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Clostridium cellulovorans |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | - |
Clostridium cellulovorans |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 8 | when incubated at 40°C for 12 h with no substrate | Clostridium cellulovorans |
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