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Literature summary for 3.2.1.74 extracted from

  • Jagtap, S.; Rao, M.
    Purification and properties of a low molecular weight 1,4-beta-d-glucan glucohydrolase having one active site for carboxymethyl cellulose and xylan from an alkalothermophilic Thermomonospora sp (2005), Biochem. Biophys. Res. Commun., 329, 111-116.
    View publication on PubMed

Inhibitors

Inhibitors Comment Organism Structure
o-phthaldialdehyde
-
Thermomonospora sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Thermomonospora sp.
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
14200
-
x * 14200, SDS-PAGE Thermomonospora sp.

Organism

Organism UniProt Comment Textmining
Thermomonospora sp.
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
Thermomonospora sp.

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
Thermomonospora sp.
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
58.79
-
carboxymethylcellulase activity Thermomonospora sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethylcellulose + H2O
-
Thermomonospora sp. D-glucose + cellooligosaccharides
-
?
additional information the enzyme exhibits both endocarboxymethyl cellulase and endoxylanase activity, both substrates are hydrolyzed at different active sites Thermomonospora sp. ?
-
?

Subunits

Subunits Comment Organism
? x * 14200, SDS-PAGE Thermomonospora sp.

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Thermomonospora sp.

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
15 h, enzyme retains complete activity of hydrolyzing xylan and carboxymethylcellulose Thermomonospora sp.
80
-
half-life: 20 min Thermomonospora sp.

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
for both cellulase and xylanase activity Thermomonospora sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
5 8 50°C, 1 h, less than 15% loss of activity Thermomonospora sp.

pI Value

Organism Comment pI Value Maximum pI Value
Thermomonospora sp. isoelectric focusing
-
4.33