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Literature summary for 3.2.1.74 extracted from
- Structural Basis for the exocellulase activity of the cellobiohydrolase CbhA from Clostridium thermocellum (2004), Biochemistry, 43, 1163-1170.
Application
| Application | Comment | Organism |
|---|---|---|
| additional information | obtaining of renewable energy | Acetivibrio thermocellus |
Crystallization (Commentary)
| Crystallization (Comment) | Organism |
|---|---|
| combination of the immunoglobulin-like module and the catalytic module and complex of inactive mutant with cellotetraose, modified microbatch crystallization under oil at 0ºC | Acetivibrio thermocellus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| E795Q | inactive enzyme | Acetivibrio thermocellus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | Acetivibrio thermocellus | nutritional resource | ? | - |
? |  |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Acetivibrio thermocellus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | - |
Acetivibrio thermocellus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by nickel affinity chromatography and gel filtration chromatography | Acetivibrio thermocellus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| acid-swollen cellulose + H2O | - |
Acetivibrio thermocellus | cellobiose | - |
? | |
| avicel + H2O | - |
Acetivibrio thermocellus | cellobiose | - |
? | |
| cellulose + H2O | nutritional resource | Acetivibrio thermocellus | ? | - |
? | |
| filter paper + H2O | - |
Acetivibrio thermocellus | cellobiose + ? | - |
? | |
| p-nitrophenyl-cellobioside + H2O | - |
Acetivibrio thermocellus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| CbhA | - |
Acetivibrio thermocellus |
| cellobiohydrolase | - |
Acetivibrio thermocellus |
| exocellulase | - |
Acetivibrio thermocellus |
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Release 2023.1 (January 2023)
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