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Literature summary for 3.2.1.74 extracted from
- Purification and some properties of exo-1,4-beta-glucanase from Chaetomium olivaceum (2003), Acta Microbiol. Pol., 52, 35-44.
Application
| Application | Comment | Organism |
|---|---|---|
| biotechnology | hydrolysis of lignocellulosic materials | Chaetomium globosum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ag+ | 5 min at 50ºC, 10mM, 100.0% of inhibition; 5 min at 50ºC, 1 mM, 100.0% inhibition | Chaetomium globosum |  |
| Ca2+ | 5 min at 50ºC, 10 mM, 15.9% inhibition; 5 min at 50ºC, 1 mM, 12.5% inhibition | Chaetomium globosum | |
| Cu2+ | 5 min at 50ºC, 10 mM, 72.0% inhibition; 5 min at 50ºC, 1 mM, 67.8% inhibition | Chaetomium globosum | |
| Hg2+ | 5 min at 50ºC, 10 mM, 100.0% inhibition; 5 min at 50ºC, 1 mM, 100.0% inhibition | Chaetomium globosum | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.65 | - |
alpha-cellulose | pH 5.0 | Chaetomium globosum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| soluble | - |
Chaetomium globosum | - |
- |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | 5 min at 50ºC, 1 mM, 103.0% of the relative activity without metal ions | Chaetomium globosum | |
| Co2+ | 5 min at 50ºC, 10 mM, 101.9% of the relative activity without metal ions | Chaetomium globosum | |
| Fe3+ | 5 min at 50ºC, 1 mM, 105.5% of the relative activity without metal ions | Chaetomium globosum | |
| Fe3+ | 5 min at 50ºC, 10 mM, 103.6% of the relative activity without metal ions | Chaetomium globosum | |
| Mn2+ | 5 min at 50ºC, 1 mM, 105.8% of the relative activity without metal ions | Chaetomium globosum | |
| Mn2+ | 5 min at 50ºC, 10 mM, 104.1% of the relative activity without metal ions | Chaetomium globosum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 88000 | - |
SDS-PAGE | Chaetomium globosum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellulose + H2O | Chaetomium globosum | principal role in cellulolysis | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Chaetomium globosum | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| no modification | - |
Chaetomium globosum |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| precipitation with acetone, gel filtration chromatography on Sephadex G-100 and DEAE-cellulose chromatography, 47.14fold | Chaetomium globosum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.9 | - |
sigmacell, pH 5.0 | Chaetomium globosum |
| 2.53 | - |
cellulose acetate, pH 5.0 | Chaetomium globosum |
| 3.36 | - |
cellulose, pH 5.0 | Chaetomium globosum |
| 3.583 | - |
alpha-cellulose, pH 5.0 | Chaetomium globosum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| alpha-cellulose + H2O | no activity with carboxymethylcellulose, salicin, cellobiose and alpha-dimethylglucoside | Chaetomium globosum | ? | - |
? | |
| cellulose + H2O | - |
Chaetomium globosum | ? | - |
? | |
| cellulose + H2O | principal role in cellulolysis | Chaetomium globosum | ? | - |
? | |
| cellulose acetate + H2O | - |
Chaetomium globosum | ? | - |
? | |
| sigmacell + H2O | - |
Chaetomium globosum | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| exo-1,4-beta-glucanase | - |
Chaetomium globosum |
| exoglucanase | - |
Chaetomium globosum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 5.2 | Chaetomium globosum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 45 | for 1 h, pH 5.2 | Chaetomium globosum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.2 | - |
at 50ºC | Chaetomium globosum |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5 | 5.5 | for 1 h, at 50ºC | Chaetomium globosum |
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