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Literature summary for 3.2.1.74 extracted from
- Characterization of the gene celD and its encoded product 1,4-beta-D-glucan glucohydrolase D from Pseudomonas fluorescens subsp. cellulosa (1992), Biochem. J., 285, 947-955.
No PubMed abstract available
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| Escherichia coli | Pseudomonas fluorescens |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.028 | - |
cellohexaose | - |
Pseudomonas fluorescens |  |
| 1.2 | - |
cellobiose | - |
Pseudomonas fluorescens | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| cell envelope | expressed in Escherichia coli | Pseudomonas fluorescens | 30313 | - |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 80000 | - |
x * 80000, SDS-PAGE | Pseudomonas fluorescens |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas fluorescens | - |
subsp. cellulosa | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 4-methylumbelliferyl beta-cellobioside + H2O | - |
Pseudomonas fluorescens | 4-methylumbelliferone + cellobiose | - |
? | |
| 4-methylumbelliferyl beta-D-glucoside + H2O | - |
Pseudomonas fluorescens | 4-methylumbelliferone + D-glucopyranose | - |
? | |
| cellobiose + H2O | - |
Pseudomonas fluorescens | D-glucose + cellotetraose + cellotriose | D-glucose only | ? | |
| cellohexaose + H2O | - |
Pseudomonas fluorescens | D-glucose + ? | in the early stages also cellopentaose and cellotetraose | ? | |
| cellopentaose + H2O | - |
Pseudomonas fluorescens | D-glucose + ? | - |
? | |
| cellotetraose + H2O | - |
Pseudomonas fluorescens | D-glucose + cellotriose + cellobiose | - |
? | |
| cellotriose + H2O | - |
Pseudomonas fluorescens | D-glucose + cellobiose | D-glucose only | ? | |
| p-nitrophenyl-beta-D-cellobioside + H2O | - |
Pseudomonas fluorescens | D-glucose + p-nitrophenyl-beta-D-glucoside | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, SDS-PAGE | Pseudomonas fluorescens |
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