Cloned (Comment) | Organism |
---|---|
Escherichia coli and Streptomyces lividans | Thermobifida fusca |
Protein Variants | Comment | Organism |
---|---|---|
A229V | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
E495D | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
E495N | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
G234A | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
G234D | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
G234N | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
G234S | loop mutant, higher binding affinities for 4-methylumbelliferyl beta-cellotrioside and cellotriose | Thermobifida fusca |
G234S/G284P | loop mutant, higher binding affinity for 4-methylumbelliferyl beta-cellotrioside | Thermobifida fusca |
G284A | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
G284I | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
G284P | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
G284P/A229V | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
G331A | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
G496P | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
H326A | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
H326S | active site mutant, higher binding affinities for 4-methylumbelliferyl beta-cellobioside and cellotriose | Thermobifida fusca |
L230C | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
L230C/D512C | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
N233C | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
N233C/D506C | loop mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
W329C | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
W332A | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
W332Y | active site mutant, weaker binding affinities to all tested ligands | Thermobifida fusca |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-cellobiose | competitive inhibition | Thermobifida fusca |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Thermobifida fusca | - |
- |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Thermobifida fusca | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture medium | - |
Thermobifida fusca | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methylumbelliferyl 4-O-(beta-D-glucopyranosyl)-beta-D-xylopyranoside + H2O | - |
Thermobifida fusca | ? | - |
? | |
4-methylumbelliferyl beta-cellobioside + H2O | - |
Thermobifida fusca | 4-methylumbelliferone + cellobiose | - |
? | |
4-methylumbelliferyl beta-cellotrioside + H2O | - |
Thermobifida fusca | 4-methylumbelliferone + cellotriose | - |
? | |
4-methylumbelliferyl beta-D-glucopyranoside + H2O | - |
Thermobifida fusca | 4-methylumbelliferone + D-glucopyranose | - |
? | |
carboxymethyl cellulose + H2O | - |
Thermobifida fusca | ? | - |
? | |
cellopentaose + H2O | - |
Thermobifida fusca | D-glucose + ? | - |
? | |
cellotetraose + H2O | - |
Thermobifida fusca | D-glucose + cellotriose + cellobiose | - |
? | |
cellotriose + H2O | - |
Thermobifida fusca | D-glucose + cellobiose | - |
? | |
cellulose + H2O | H3PO4 swollen | Thermobifida fusca | D-glucose + ? | - |
? | |
cellulose + H2O | bacterial microcrystalline cellulose | Thermobifida fusca | D-glucose + ? | - |
? |