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Literature summary for 3.2.1.74 extracted from
- beta-Glucosidase from Ruminococcus albus (1988), Methods Enzymol., 160, 408-414.
No PubMed abstract available
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Ruminococcus albus |  |
| Hg2+ | - |
Ruminococcus albus | |
| iodoacetoamide | - |
Ruminococcus albus | |
| p-chloromercuribenzoate | - |
Ruminococcus albus | |
| Zn2+ | - |
Ruminococcus albus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 2.2 | - |
p-nitrophenyl-beta-D-glucoside | - |
Ruminococcus albus | |
| 26 | - |
cellobiose | - |
Ruminococcus albus | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Ruminococcus albus | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| 520fold | Ruminococcus albus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 13 | - |
- |
Ruminococcus albus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| cellobiose + H2O | - |
Ruminococcus albus | D-glucose + cellotetraose + cellotriose | - |
ir | |
| cellooligomers + H2O | - |
Ruminococcus albus | D-glucose + cellooligomers | - |
ir | |
| cellopentaose + H2O | - |
Ruminococcus albus | D-glucose + ? | - |
ir | |
| cellotetraose + H2O | - |
Ruminococcus albus | D-glucose + cellotriose + cellobiose | - |
ir | |
| cellotriose + H2O | - |
Ruminococcus albus | D-glucose + cellobiose | - |
ir | |
| p-nitrophenyl-beta-D-cellobioside + H2O | - |
Ruminococcus albus | D-glucose + p-nitrophenyl-beta-D-glucoside | - |
? | |
| p-nitrophenyl-beta-D-glucopyranoside + H2O | - |
Ruminococcus albus | 4-nitrophenol + D-glucose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 35 | - |
Ruminococcus albus |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
stable below 30°C | Ruminococcus albus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6.5 | - |
- |
Ruminococcus albus |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 7.5 | most stable at pH 6-7.5 | Ruminococcus albus |
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