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Literature summary for 3.2.1.74 extracted from

  • Himmel, M.E.; Tucker, M.P.; Lastick, S.M.; Oh, K.K.; Fox, J.W.; Spindler, D.D.; Grohmann, K.
    Isolation and characterization of a 1,4-beta-D-glucan glucohydrolase from the yeast, Torulopsis wickerhamii (1986), J. Biol. Chem., 261, 12948-12955.
    View publication on PubMed
Search for more literature for 3.2.1.74

Inhibitors

Inhibitors Comment Organism Structure
D-cellobiose competitive inhibition Nakazawaea wickerhamii
D-glucose competitive inhibition Nakazawaea wickerhamii

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.8
-
 p-nitrophenyl-beta-D-glucopyranoside
-
 Nakazawaea wickerhamii

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Nakazawaea wickerhamii
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83500
-
 2 * 83500 Nakazawaea wickerhamii
151000
-
 gel filtration Nakazawaea wickerhamii

Organism

Organism UniProt Comment Textmining
Nakazawaea wickerhamii
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 Nakazawaea wickerhamii

Source Tissue

Source Tissue Comment Organism Textmining
culture medium
-
 Nakazawaea wickerhamii
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
276.7
-
-
 Nakazawaea wickerhamii

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
beta-glucan + H2O from yeast and from barley Nakazawaea wickerhamii ?
-
 ?
cellobiose + H2O
-
 Nakazawaea wickerhamii D-glucose + cellotetraose + cellotriose
-
 ?
celloheptaose + H2O
-
 Nakazawaea wickerhamii ?
-
 ?
cellohexaose + H2O
-
 Nakazawaea wickerhamii D-glucose + ?
-
 ?
cellopentaose + H2O
-
 Nakazawaea wickerhamii D-glucose + ?
-
 ?
cellotriose + H2O
-
 Nakazawaea wickerhamii D-glucose + cellobiose
-
 ?
gentiobiose + H2O
-
 Nakazawaea wickerhamii ?
-
 ?
hydroquinone-beta-D-glucopyranoside + H2O
-
 Nakazawaea wickerhamii hydroquinone + D-glucose
-
 ?
laminaribiose + H2O
-
 Nakazawaea wickerhamii ?
-
 ?
laminaritriose + H2O from barley Nakazawaea wickerhamii ?
-
 ?
n-heptyl-beta-D-glucopyranoside + H2O
-
 Nakazawaea wickerhamii n-heptanol + D-glucose
-
 ?
n-hexyl-beta-D-glucopyranoside + H2O
-
 Nakazawaea wickerhamii n-hexanol + D-glucose
-
 ?
n-octyl-beta-D-glucopyranoside + H2O
-
 Nakazawaea wickerhamii n-octanol + D-glucose
-
 ?
n-pentyl-beta-D-glucopyranoside + H2O
-
 Nakazawaea wickerhamii n-pentanol + D-glucose
-
 ?
p-nitrophenyl-beta-D-glucopyranoside + H2O
-
 Nakazawaea wickerhamii 4-nitrophenol + D-glucose
-
 ?
phenyl-beta-D-glucopyranoside + H2O
-
 Nakazawaea wickerhamii phenol + D-glucose
-
 ?
sophorose + H2O
-
 Nakazawaea wickerhamii ?
-
 ?
xylobiose + H2O
-
 Nakazawaea wickerhamii ?
-
 ?

Subunits

Subunits Comment Organism
dimer 2 * 83500 Nakazawaea wickerhamii

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
 Nakazawaea wickerhamii

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
55
-
 stable below 55°C Nakazawaea wickerhamii

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.3
-
-
 Nakazawaea wickerhamii