Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 3.2.1.73 extracted from

  • Jung, Y.J.; Lee, Y.S.; Park, I.H.; Chandra, M.S.; Kim, K.K.; Choi, Y.L.
    Molecular cloning, purification and characterization of thermostable beta-1,3-1,4 glucanase from Bacillus subtilis A8-8 (2010), Indian J. Biochem. Biophys., 47, 203-210.
    View publication on PubMed
Search for more literature for 3.2.1.73

Cloned(Commentary)

Cloned (Comment) Organism
expression as GST-fusion protein Bacillus subtilis

Protein Variants

Protein Variants Comment Organism
additional information the truncated gene product, devoid of cellulose-binding domain, shows 60% of activity and binds to avicel Bacillus subtilis

Inhibitors

Inhibitors Comment Organism Structure
Fe3+ 5 mM, 58% of initial activity Bacillus subtilis
additional information not inhibitory: EDTA Bacillus subtilis

Metals/Ions

Metals/Ions Comment Organism Structure
Co2+ 5 mM, 160% of initial activity Bacillus subtilis
Mn2+ 5 mM, 152% of initial activity Bacillus subtilis

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
 x * 55000, SDS-PAGE Bacillus subtilis

Organism

Organism UniProt Comment Textmining
Bacillus subtilis
-
-
-
Bacillus subtilis A8-8
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl beta-D-cellobioside + H2O 1% of the activity with carboxymethyl-cellulose Bacillus subtilis ?
-
 ?
avicel + H2O 50% of the activity with carboxymethyl-cellulose Bacillus subtilis ?
-
 ?
avicel + H2O 50% of the activity with carboxymethyl-cellulose Bacillus subtilis A8-8 ?
-
 ?
carboxymethyl-cellulose + H2O
-
 Bacillus subtilis ?
-
 ?
laminarin + H2O 70% of the activity with carboxymethyl-cellulose Bacillus subtilis ?
-
 ?
laminarin + H2O 70% of the activity with carboxymethyl-cellulose Bacillus subtilis A8-8 ?
-
 ?
lichenan + H2O 500% of the activity with carboxymethyl-cellulose Bacillus subtilis ?
-
 ?
lichenan + H2O 500% of the activity with carboxymethyl-cellulose Bacillus subtilis A8-8 ?
-
 ?
additional information no substrate: cellobiose Bacillus subtilis ?
-
 ?
additional information no substrate: cellobiose Bacillus subtilis A8-8 ?
-
 ?
xylan + H2O 500% of the activity with carboxymethyl-cellulose Bacillus subtilis ?
-
 ?
xylan + H2O 500% of the activity with carboxymethyl-cellulose Bacillus subtilis A8-8 ?
-
 ?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Bacillus subtilis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
60
-
-
 Bacillus subtilis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Bacillus subtilis

pH Stability

pH Stability pH Stability Maximum Comment Organism
6 9 stable within Bacillus subtilis