Activating Compound | Comment | Organism | Structure |
---|---|---|---|
additional information | structural lid (acids 99-97) at the active site generated by the tetramerization is closely associated with the bifunctionality and in particular with the alpha-1,4-transferase activity | Saccharolobus solfataricus |
Application | Comment | Organism |
---|---|---|
additional information | bifunctional mechanism of the archaeal glycogen-debranching enzyme TreX, showing alpha-1,4-transferase and alpha-1,6-glucosidase activities | Saccharolobus solfataricus |
Crystallization (Comment) | Organism |
---|---|
at a resolution of 2.8 A, TreX crystallized in the dimeric and tetrameric forms, in space groups P3121 and P321, respectively and in complex with an acarbose ligand. Acarbose intermediate is covalently bound to Asp363, occupying subsites -1 to -3 | Saccharolobus solfataricus |
Protein Variants | Comment | Organism |
---|---|---|
D318A | shows an activity level identical to that of the wild-type | Saccharolobus solfataricus |
E94A | shows a relatively minor change in the alpha-1,6-glucosidase activity, but a sharply increased alpha-1,4-transferase activity compared with the wild-type | Saccharolobus solfataricus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Saccharolobus solfataricus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glycogen + H2O | - |
Saccharolobus solfataricus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
dimer | crystallography | Saccharolobus solfataricus |
tetramer | crystallography | Saccharolobus solfataricus |
Synonyms | Comment | Organism |
---|---|---|
alpha-1,6-glucosidase | - |
Saccharolobus solfataricus |
TreX | - |
Saccharolobus solfataricus |