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Literature summary for 3.2.1.70 extracted from

  • Woo, E.J.; Lee, S.; Cha, H.; Park, J.T.; Yoon, S.M.; Song, H.N.; Park, K.H.
    Structural Insight into the Bifunctional Mechanism of the Glycogen-debranching Enzyme TreX from the Archaeon Sulfolobus solfataricus (2008), J. Biol. Chem., 283, 28641-28648.
    View publication on PubMedView publication on EuropePMC

Activating Compound

Activating Compound Comment Organism Structure
additional information structural lid (acids 99-97) at the active site generated by the tetramerization is closely associated with the bifunctionality and in particular with the alpha-1,4-transferase activity Saccharolobus solfataricus

Application

Application Comment Organism
additional information bifunctional mechanism of the archaeal glycogen-debranching enzyme TreX, showing alpha-1,4-transferase and alpha-1,6-glucosidase activities Saccharolobus solfataricus

Crystallization (Commentary)

Crystallization (Comment) Organism
at a resolution of 2.8 A, TreX crystallized in the dimeric and tetrameric forms, in space groups P3121 and P321, respectively and in complex with an acarbose ligand. Acarbose intermediate is covalently bound to Asp363, occupying subsites -1 to -3 Saccharolobus solfataricus

Protein Variants

Protein Variants Comment Organism
D318A shows an activity level identical to that of the wild-type Saccharolobus solfataricus
E94A shows a relatively minor change in the alpha-1,6-glucosidase activity, but a sharply increased alpha-1,4-transferase activity compared with the wild-type Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glycogen + H2O
-
Saccharolobus solfataricus ?
-
?

Subunits

Subunits Comment Organism
dimer crystallography Saccharolobus solfataricus
tetramer crystallography Saccharolobus solfataricus

Synonyms

Synonyms Comment Organism
alpha-1,6-glucosidase
-
Saccharolobus solfataricus
TreX
-
Saccharolobus solfataricus