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Literature summary for 3.2.1.7 extracted from

  • Tian, F.; Khodadadi, M.; Karboune, S.
    Optimization of levansucrase/endo-inulinase bi-enzymatic system for the production of fructooligosaccharides and oligolevans from sucrose (2014), J. Mol. Catal. B, 109, 85-93.
No PubMed abstract available

Application

Application Comment Organism
synthesis application of a bi-enzymatic system based on the combined use of levansucrase from Bacillus amyloliquefaciens and endo-inulinase from Aspergillus niger in a one-step reaction for the synthesis of fructooligosaccharides and oligolevans using sucrose as the sole substrate. The optimal conditions leading to a high yield of short chain fructooligosaccharides, i.e.1:1 ratio, 0.5 h, 0.6 M, are different from those resulting in a high yield of medium chain fructooligosaccharides and oligolevans, i.e. 1.85:1 ratio, 1.77 h, 0.6 M. The production of fructooligosaccharides and oligolevans at a large scale gives a yield of 57-65%, w/w and produces 65.8-266.8 g/l and h, and uses of low temperature of 35°C and low concentrations of sucrose Aspergillus niger

Organism

Organism UniProt Comment Textmining
Aspergillus niger
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