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Literature summary for 3.2.1.68 extracted from

  • Fang, T.; Tseng, W.; Yu, C.; Shih, T.
    Characterization of the thermophilic isoamylase from the thermophilic archaeon Sulfolobus solfataricus ATCC 35092 (2005), J. Mol. Catal. B, 33, 99-107.
No PubMed abstract available
Search for more literature for 3.2.1.68

Application

Application Comment Organism
degradation thermophilic enzyme shows a potential to be used in industry to degrade the debranching points of starch at a high temperature Saccharolobus solfataricus

Cloned(Commentary)

Cloned (Comment) Organism
cloned to an expression vector with a T7lac promoter. Both wild-type and His-tagged isoamylases are expressed in Escherichia coli Saccharolobus solfataricus

Organism

Organism UniProt Comment Textmining
Saccharolobus solfataricus
-
 ATCC 35092
-

Purification (Commentary)

Purification (Comment) Organism
recombinant wild-type and His-tagged enzyme Saccharolobus solfataricus

Subunits

Subunits Comment Organism
trimer
-
 Saccharolobus solfataricus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
75
-
-
 Saccharolobus solfataricus

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
4
-
 recombinant wild-type and His-tagged enzyme are more stable at room temperature than at 4°C Saccharolobus solfataricus
21
-
 recombinant wild-type and His-tagged enzyme are more stable at room temperature than at 4°C Saccharolobus solfataricus
75
-
 2 h, recombinant wild-type enzyme and His-tagged enzyme are sstable Saccharolobus solfataricus
80
-
 2 h, recombinant wild-type enzyme and His-tagged enzyme are sstable Saccharolobus solfataricus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Saccharolobus solfataricus