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Literature summary for 3.2.1.68 extracted from

  • Krohn, M.; Barry, G.F.; Kishore, G.M.
    An isoamylase with neutral pH optimum from a Flavobacterium species: cloning, characterization and expression of the iam gene (1997), Mol. Gen. Genet., 254, 469-478.
    View publication on PubMed

Application

Application Comment Organism
analysis
-
Pseudomonas amyloderamosa
nutrition industrial production of various syrups from starch Flavobacterium sp.

Cloned(Commentary)

Cloned (Comment) Organism
iam gene cloned and expressed in Escherichia coli JM101, pMON17481, production of a nonsecreted signal peptide-less isoamylase Flavobacterium sp.

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Flavobacterium sp.
-
-
extracellular
-
Pseudomonas amyloderamosa
-
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
83000
-
SDS-PAGE, co-purified with a second minor band of 75000, probably C-terminal truncation of the native enzyme in vivo or during purification Flavobacterium sp.
86000 94000
-
Pseudomonas amyloderamosa

Organism

Organism UniProt Comment Textmining
Flavobacterium sp.
-
-
-
Pseudomonas amyloderamosa
-
isoamylase-hyperproducing mutant
-
Pseudomonas amyloderamosa
-
SMP1
-
Pseudomonas amyloderamosa JD210
-
isoamylase-hyperproducing mutant
-
Pseudomonas amyloderamosa SMP1
-
SMP1
-

Purification (Commentary)

Purification (Comment) Organism
-
Flavobacterium sp.

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3
-
pullulan, recombinant isoamylase Flavobacterium sp.
50
-
-
Flavobacterium sp.
54.6
-
amylose, recombinant isoamylase Flavobacterium sp.
120
-
potato starch, recombinant isoamylase Flavobacterium sp.
154
-
rice starch, recombinant isoamylase Flavobacterium sp.
172
-
corn amylopectin, recombinant isoamylase Flavobacterium sp.
174
-
rabbit liver glycogen, recombinant isoamylase Flavobacterium sp.
182
-
oyster glycogen, recombinant isoamylase Flavobacterium sp.

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
maltose + H2O iam gene induced by maltose Flavobacterium sp. ?
-
?
maltose + H2O iam gene induced by maltose Pseudomonas amyloderamosa ?
-
?
maltose + H2O iam gene induced by maltose Pseudomonas amyloderamosa JD210 ?
-
?
maltose + H2O iam gene induced by maltose Pseudomonas amyloderamosa SMP1 ?
-
?

Synonyms

Synonyms Comment Organism
bacterial isoamylase
-
Pseudomonas amyloderamosa

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
3 4
-
Pseudomonas amyloderamosa
6.3
-
only isoamylase with neutral pH optimum Flavobacterium sp.

pH Range

pH Minimum pH Maximum Comment Organism
6 7
-
Flavobacterium sp.

pH Stability

pH Stability pH Stability Maximum Comment Organism
6.5 7 assayed at 22°C enzyme displays broader activity and stability optima of pH 5.0 -7.5 Flavobacterium sp.