Application | Comment | Organism |
---|---|---|
analysis | - |
Pseudomonas amyloderamosa |
nutrition | industrial production of various syrups from starch | Flavobacterium sp. |
Cloned (Comment) | Organism |
---|---|
iam gene cloned and expressed in Escherichia coli JM101, pMON17481, production of a nonsecreted signal peptide-less isoamylase | Flavobacterium sp. |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Flavobacterium sp. | - |
- |
extracellular | - |
Pseudomonas amyloderamosa | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
83000 | - |
SDS-PAGE, co-purified with a second minor band of 75000, probably C-terminal truncation of the native enzyme in vivo or during purification | Flavobacterium sp. |
86000 | 94000 | - |
Pseudomonas amyloderamosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Flavobacterium sp. | - |
- |
- |
Pseudomonas amyloderamosa | - |
isoamylase-hyperproducing mutant | - |
Pseudomonas amyloderamosa | - |
SMP1 | - |
Pseudomonas amyloderamosa JD210 | - |
isoamylase-hyperproducing mutant | - |
Pseudomonas amyloderamosa SMP1 | - |
SMP1 | - |
Purification (Comment) | Organism |
---|---|
- |
Flavobacterium sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
3 | - |
pullulan, recombinant isoamylase | Flavobacterium sp. |
50 | - |
- |
Flavobacterium sp. |
54.6 | - |
amylose, recombinant isoamylase | Flavobacterium sp. |
120 | - |
potato starch, recombinant isoamylase | Flavobacterium sp. |
154 | - |
rice starch, recombinant isoamylase | Flavobacterium sp. |
172 | - |
corn amylopectin, recombinant isoamylase | Flavobacterium sp. |
174 | - |
rabbit liver glycogen, recombinant isoamylase | Flavobacterium sp. |
182 | - |
oyster glycogen, recombinant isoamylase | Flavobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
maltose + H2O | iam gene induced by maltose | Flavobacterium sp. | ? | - |
? | |
maltose + H2O | iam gene induced by maltose | Pseudomonas amyloderamosa | ? | - |
? | |
maltose + H2O | iam gene induced by maltose | Pseudomonas amyloderamosa JD210 | ? | - |
? | |
maltose + H2O | iam gene induced by maltose | Pseudomonas amyloderamosa SMP1 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bacterial isoamylase | - |
Pseudomonas amyloderamosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
3 | 4 | - |
Pseudomonas amyloderamosa |
6.3 | - |
only isoamylase with neutral pH optimum | Flavobacterium sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 7 | - |
Flavobacterium sp. |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7 | assayed at 22°C enzyme displays broader activity and stability optima of pH 5.0 -7.5 | Flavobacterium sp. |