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Literature summary for 3.2.1.67 extracted from

  • Mata-Gomez, M.A.; Heerd, D.; Oyanguren-Garcia, I.; Barbero, F.; Rito-Palomares, M.; Fernandez-Lahore, M.
    A novel pectin-degrading enzyme complex from Aspergillus sojae ATCC 20235 mutants (2015), J. Sci. Food Agric., 95, 1554-1561.
    View publication on PubMed
Search for more literature for 3.2.1.67

Application

Application Comment Organism
synthesis mutant strain M3 can be used for production of exopolygalacturonase Aspergillus sojae

Protein Variants

Protein Variants Comment Organism
additional information construction of mutant strain M3 with increased enzyme activity Aspergillus sojae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->4)-alpha-D-galacturonide]n + H2O Aspergillus sojae
-
 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
[(1->4)-alpha-D-galacturonide]n + H2O Aspergillus sojae ATCC 20235
-
 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Organism

Organism UniProt Comment Textmining
Aspergillus sojae
-
-
-
Aspergillus sojae ATCC 20235
-
-
-

Source Tissue

Source Tissue Comment Organism Textmining
additional information solid-state fermentation Aspergillus sojae
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 with pectin as substrate: wild-type strain 97.8 U/ml, mutant strain M3 105.0 U/ml, mutant strain GH56 105.2 U/ml, and mutant strain Guserbiot 2.230 95.1 U/ml. Mutant strain M3 yields of enzyme activity is 1111.7 U/g on sugar beet and 142.1 U/g on apple pomace, while the wild-type strain gives 718.1 U/g and 240.0 U/g for sugar beet and apple pomace, respectively. Comprisons with commercial pectinases, overview Aspergillus sojae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
pectin + H2O
-
 Aspergillus sojae ?
-
 ?
pectin + H2O
-
 Aspergillus sojae ATCC 20235 ?
-
 ?
polygalacturonic acid + H2O
-
 Aspergillus sojae ?
-
 ?
polygalacturonic acid + H2O
-
 Aspergillus sojae ATCC 20235 ?
-
 ?
[(1->4)-alpha-D-galacturonide]n + H2O
-
 Aspergillus sojae [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?
[(1->4)-alpha-D-galacturonide]n + H2O
-
 Aspergillus sojae ATCC 20235 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
 ?

Synonyms

Synonyms Comment Organism
Exo-PG
-
 Aspergillus sojae
exo-polygalacturonase
-
 Aspergillus sojae

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
25
-
 assay at Aspergillus sojae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.8
-
 assay at Aspergillus sojae