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Literature summary for 3.2.1.67 extracted from

  • Maller, A.; da Silva, T.M.; Damasio, A.R.; Hirata, I.Y.; Jorge, J.A.; Terenzi, H.F.; Polizeli, M.
    Functional properties of a manganese-activated exo-polygalacturonase produced by a thermotolerant fungus Aspergillus niveus (2013), Folia Microbiol. (Praha), 58, 615-621.
    View publication on PubMed

General Stability

General Stability Organism
anion exchange resins stabilize the enzyme Aspergillus niveus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information 6.7 mg/ml with [(1->4)-alpha-D-galacturonide]n, pH 4.0, 50°C Aspergillus niveus

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
Aspergillus niveus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Br- activates by 10% at 10 mg/ml Aspergillus niveus
F- activates by 17% at 10 mg/ml Aspergillus niveus
K+ activates by 17% at 10 mg/ml Aspergillus niveus
Mn2+ activates by 232% at 10 mg/ml Aspergillus niveus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
102600
-
x * 102600, SDS-PAGE Aspergillus niveus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[(1->4)-alpha-D-galacturonide]n + H2O Aspergillus niveus
-
[(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]n + H2O Aspergillus niveus RP05
-
[(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
?

Organism

Organism UniProt Comment Textmining
Aspergillus niveus
-
isolated from decomposing materials
-
Aspergillus niveus RP05
-
isolated from decomposing materials
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycoprotein with 37.7% carbohydrate Aspergillus niveus

Purification (Commentary)

Purification (Comment) Organism
native extracellular enzyme 4.4fold by anion exchange chromatography and affinity chromatography Aspergillus niveus

Source Tissue

Source Tissue Comment Organism Textmining
additional information highest enzyme production at 40°C Aspergillus niveus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
[(1->4)-alpha-D-galacturonide]n + H2O
-
Aspergillus niveus [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]n + H2O polypectate Aspergillus niveus [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]n + H2O
-
Aspergillus niveus RP05 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
?
[(1->4)-alpha-D-galacturonide]n + H2O polypectate Aspergillus niveus RP05 [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate
-
?

Subunits

Subunits Comment Organism
? x * 102600, SDS-PAGE Aspergillus niveus

Synonyms

Synonyms Comment Organism
Exo-PG
-
Aspergillus niveus
exo-polygalacturonase
-
Aspergillus niveus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
50
-
-
Aspergillus niveus

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
393.3
-
[(1->4)-alpha-D-galacturonide]n pH 4.0, 50°C Aspergillus niveus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4 6.5
-
Aspergillus niveus

pH Stability

pH Stability pH Stability Maximum Comment Organism
3 9 purified enzyme, 24 h, stable, mainly at pH 6.5, retaining more than 70% activity at pH 3.0 and 85% at pH 8.0 Aspergillus niveus

pI Value

Organism Comment pI Value Maximum pI Value
Aspergillus niveus
-
-
5.4

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
additional information
-
additional information 58.7 mg/ml/s with [(1->4)-alpha-D-galacturonide]n, pH 4.0, 50°C Aspergillus niveus