Protein Variants | Comment | Organism |
---|---|---|
D210N | isozyme RPG15 mutant, the mutant shows a 5000fold reduced specific activity relative to the wild-type, rendering the enzyme essentially inactive | Rhizopus arrhizus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
D-galacturonate | product inhibition | Rhizopus arrhizus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | thermodynamic parameters of a series of galacturonic acid oligomers binding to isozyme RPG15, overview | Rhizopus arrhizus | |
0.0092 | - |
[(1->4)-alpha-D-galacturonide]3 | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus | |
0.016 | - |
[(1->4)-alpha-D-galacturonide]2 | isozyme RPG16, pH 4.0, 30°C | Rhizopus arrhizus | |
0.055 | - |
[(1->4)-alpha-D-galacturonide]2 | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-galacturonide]n + H2O | Rhizopus arrhizus | - |
[(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizopus arrhizus | Q5W9U0 | two isozymes RPG15 and RPG16 | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
[(1->4)-alpha-D-galacturonide]2 + H2O | - |
Rhizopus arrhizus | 2 D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]3 + H2O | - |
Rhizopus arrhizus | [(1->4)-alpha-D-galacturonide]2 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | - |
Rhizopus arrhizus | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? | |
[(1->4)-alpha-D-galacturonide]n + H2O | the two Rhizopus oryzae exo-polygalacturonase isozymes act on galacturonic acid oligomers, isothermal titration calorimetry analysis, overview | Rhizopus arrhizus | [(1->4)-alpha-D-galacturonide]n-1 + D-galacturonate | - |
? |
Synonyms | Comment | Organism |
---|---|---|
Exo-PG | - |
Rhizopus arrhizus |
exo-polygalacturonase | - |
Rhizopus arrhizus |
RPG15 | - |
Rhizopus arrhizus |
RPG16 | - |
Rhizopus arrhizus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Rhizopus arrhizus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
3.9 | - |
[(1->4)-alpha-D-galacturonide]2 | isozyme RPG16, pH 4.0, 30°C | Rhizopus arrhizus | |
10.3 | - |
[(1->4)-alpha-D-galacturonide]2 | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus | |
10.7 | - |
[(1->4)-alpha-D-galacturonide]3 | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
assay at | Rhizopus arrhizus |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.501 | - |
D-galacturonate | isozyme RPG16, pH 4.0, 30°C | Rhizopus arrhizus | |
0.886 | - |
D-galacturonate | isozyme RPG15, pH 4.0, 30°C | Rhizopus arrhizus |
General Information | Comment | Organism |
---|---|---|
evolution | the enzyme belongs to the glycosyl hydrolase 28 family, GH28 | Rhizopus arrhizus |