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Literature summary for 3.2.1.67 extracted from

  • Kluskens, L.D.; van Alebeek, G.J.; Walther, J.; Voragen, A.G.; de Vos, W.M.; van der Oost, J.
    Characterization and mode of action of an exopolygalacturonase from the hyperthermophilic bacterium Thermotoga maritima (2005), FEBS J., 272, 5464-5473.
    View publication on PubMed
Search for more literature for 3.2.1.67

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Thermotoga maritima

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.06
-
 (1,4-alpha-D-galacturonide)n 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
0.06
-
 (1,4-alpha-D-galacturonide)8 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
0.07
-
 (1,4-alpha-D-galacturonide)7 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
0.11
-
 (1,4-alpha-D-galacturonide)6 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
0.24
-
 (1,4-alpha-D-galacturonide)5 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
0.29
-
 (1,4-alpha-D-galacturonide)4 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
0.34
-
 (1,4-alpha-D-galacturonide)3 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
0.34
-
 (1,4-alpha-D-galacturonide)2 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Thermotoga maritima 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
50000
-
 4 * 50000, molecular weight of the monomer deduced from sequence Thermotoga maritima
212000
-
 gel filtration Thermotoga maritima

Organism

Organism UniProt Comment Textmining
Thermotoga maritima
-
-
-

Purification (Commentary)

Purification (Comment) Organism
anion-exchange chromatography Thermotoga maritima

Source Tissue

Source Tissue Comment Organism Textmining
cell culture
-
 Thermotoga maritima
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(1,4-alpha-D-galacturonide)2 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)3 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)4 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)5 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)6 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)7 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)8 + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?
(1,4-alpha-D-galacturonide)n + H2O
-
 Thermotoga maritima D-galacturonic acid
-
 ?

Subunits

Subunits Comment Organism
tetramer 4 * 50000, molecular weight of the monomer deduced from sequence Thermotoga maritima

Synonyms

Synonyms Comment Organism
PelB
-
 Thermotoga maritima

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
80
-
-
 Thermotoga maritima

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
182
-
 (1,4-alpha-D-galacturonide)2 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
685
-
 (1,4-alpha-D-galacturonide)3 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
821
-
 (1,4-alpha-D-galacturonide)6 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
829
-
 (1,4-alpha-D-galacturonide)4 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
843
-
 (1,4-alpha-D-galacturonide)8 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
860
-
 (1,4-alpha-D-galacturonide)7 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
934
-
 (1,4-alpha-D-galacturonide)5 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima
936
-
 (1,4-alpha-D-galacturonide)n 30 mM phosphate buffer pH 6.5, 80°C Thermotoga maritima

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6.4
-
-
 Thermotoga maritima