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Literature summary for 3.2.1.67 extracted from
- Purification and characterization of two isozymes of polygalacturonase from Botrytis cinerea. Effect of calcium ions on polygalacturonase activity (2002), Microbiol. Res., 157, 183-189.
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Botrytis cinerea | - |
- |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 63000 | - |
gel filtration | Botrytis cinerea |
| 65000 | - |
1 * 65000, SDS-PAGE | Botrytis cinerea |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Botrytis cinerea | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Botrytis cinerea |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.6 | - |
pH 4.5, 30°C | Botrytis cinerea |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| (1,4-alpha-D-galacturonide)n + H2O | - |
Botrytis cinerea | D-galacturonic acid | - |
? |  |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 65000, SDS-PAGE | Botrytis cinerea |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | - |
- |
Botrytis cinerea |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 3 | 6 | - |
Botrytis cinerea |
pI Value
| Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|
| Botrytis cinerea | isoelectric focusing | - |
8 |
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