Literature summary for 3.2.1.67 extracted from

Kester, H.C.M.; Kusters-Van Someren, M.A.; Mueller, Y.; Visser, J.
Primary structure and characterization of an exopolygalacturonase from Aspergillus tubingensis (1996), Eur. J. Biochem., 240, 738-746.

Search for more literature for 3.2.1.67

Application

Application	Comment	Organism
synthesis	pectic enzymes industrially important in processing of agricultural products, production of pectinolytic enzymes, specifical enzyme for modification of pectins	Aspergillus tubingensis

Cloned(Commentary)

Cloned (Comment)	Organism
gene pgaX cloned by reverse genetics, pIM361, overexpression of the enzyme by transformants	Aspergillus tubingensis

Inhibitors

Inhibitors	Comment	Organism	Structure
D-galactopyranuronic acid	catabolite repression, completely inhibited, inhibitory constant 0.3 mM	Aspergillus tubingensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.32	-	(1,4-alpha-D-galacturonide)7	-	Aspergillus tubingensis
0.44	-	(1,4-alpha-D-galacturonide)6	-	Aspergillus tubingensis
0.53	-	(1,4-alpha-D-galacturonide)5	-	Aspergillus tubingensis
0.68	-	(1,4-alpha-D-galacturonide)3	-	Aspergillus tubingensis
0.94	-	(1,4-alpha-D-galacturonide)3	-	Aspergillus tubingensis
1.44	-	(1,4-alpha-D-galacturonide)2	-	Aspergillus tubingensis
3.1	-	(1,4-alpha-D-galacturonide)n	recombinant enzyme	Aspergillus tubingensis
3.2	-	(1,4-alpha-D-galacturonide)n	wild type enzyme	Aspergillus tubingensis

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
extracellular	-	Aspergillus tubingensis	-	-

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
78000	-	SDS-PAGE	Aspergillus tubingensis

Organism

Organism	UniProt	Comment	Textmining
Aspergillus tubingensis	-	filamentous fungus, wild-type strain	-

Purification (Commentary)

Purification (Comment)	Organism
wild-type and recombinant enzyme	Aspergillus tubingensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
(1,4-alpha-D-galacturonide)2 + H2O	-	Aspergillus tubingensis	D-galacturonic acid	-	?
(1,4-alpha-D-galacturonide)3 + H2O	-	Aspergillus tubingensis	galacturonic acid	-	?
(1,4-alpha-D-galacturonide)4 + H2O	-	Aspergillus tubingensis	D-galacturonate	-	?
(1,4-alpha-D-galacturonide)5 + H2O	-	Aspergillus tubingensis	D-galacturonic acid + (1,4-alpha-D-galacturonide)2 + (1,4-alpha-D-galacturonide)3 + (1,4-alpha-D-galacturonide)4	-	?
(1,4-alpha-D-galacturonide)6 + H2O	-	Aspergillus tubingensis	D-galacturonic acid + (1,4-alpha-D-galacturonide)5	-	?
(1,4-alpha-D-galacturonide)7 + H2O	-	Aspergillus tubingensis	?	-	?
(1,4-alpha-D-galacturonide)n + H2O	-	Aspergillus tubingensis	D-galacturonate + digalacturonic acid + trigalacturonic acid	-	?
oligo-D-galacturonate + H2O	-	Aspergillus tubingensis	D-galacturonic acid	-	?

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
30	50	50°C 50% activity retained	Aspergillus tubingensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
4.2	-	-	Aspergillus tubingensis

pH Range

pH Minimum	pH Maximum	Comment	Organism
3	5	-	Aspergillus tubingensis

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Release 2023.1 (January 2023)