Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Sodium azide | for D766A mutant only | Bifidobacterium bifidum |
Cloned (Comment) | Organism |
---|---|
overexpression in Escherichia coli, selenomethionine-substituted protein | Bifidobacterium bifidum |
Crystallization (Comment) | Organism |
---|---|
of the AfcA catalytic domain in unliganded and complexed forms with the inhibitor deoxyfuconojirimycin, the substrate 2-fucosyllactose, and the products L-fucose and lactose at 1.12-2.10 A resolution | Bifidobacterium bifidum |
Protein Variants | Comment | Organism |
---|---|---|
D766A | site directed mutagenesis | Bifidobacterium bifidum |
D766E | site directed mutagenesis | Bifidobacterium bifidum |
E485A | site directed mutagenesis | Bifidobacterium bifidum |
E566A | site directed mutagenesis | Bifidobacterium bifidum |
N421A | site directed mutagenesis | Bifidobacterium bifidum |
N421G | site directed mutagenesis | Bifidobacterium bifidum |
N421G/N423G | site directed mutagenesis | Bifidobacterium bifidum |
N423D | site directed mutagenesis | Bifidobacterium bifidum |
N423G | site directed mutagenesis | Bifidobacterium bifidum |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxyfuconojirimycin | - |
Bifidobacterium bifidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.035 | - |
2-fucosyllactose | N421A mutant | Bifidobacterium bifidum | |
0.056 | - |
2-fucosyllactose | N421G mutant | Bifidobacterium bifidum | |
0.075 | - |
2-fucosyllactose | N423G mutant | Bifidobacterium bifidum | |
0.089 | - |
2-fucosyllactose | D766A mutant | Bifidobacterium bifidum | |
0.091 | - |
2-fucosyllactose | wild type | Bifidobacterium bifidum | |
0.16 | - |
2-fucosyllactose | E566A mutant | Bifidobacterium bifidum | |
0.19 | - |
2-fucosyllactose | D766E mutant | Bifidobacterium bifidum | |
0.32 | - |
2-fucosyllactose | N423D mutant | Bifidobacterium bifidum | |
17 | - |
2-fucosyllactose | E485A mutant | Bifidobacterium bifidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
of the recombinant protein by saturated ammonium sulfate precipitation and Q sepharose fast flow, CHT ceramic hydroxyapatite and gel filtration column chromatography | Bifidobacterium bifidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-fucosyllactose + H2O | - |
Bifidobacterium bifidum | fucose + lactose | - |
? | |
methyl-2-alpha-L-fucopyranosyl-beta-D-galactoside + H2O | - |
Bifidobacterium bifidum | L-fucose + methyl beta-D-galactoside | - |
? |
Synonyms | Comment | Organism |
---|---|---|
1,2-alpha-L-fucosidase | - |
Bifidobacterium bifidum |
AfcA | from glycoside hydrolase family 95 | Bifidobacterium bifidum |