Cloned (Comment) | Organism |
---|---|
expression of 1,2-alpha-L-fucosidase in Escherichia coli, expression of the Fuc domain (responsible for fucosidase activity) as a hexahistidine-tagged protein in Escherichia coli | Bifidobacterium bifidum |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.53 | - |
2'-fucosyllactose | Fuc domain | Bifidobacterium bifidum |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
205000 | - |
x * 205000, calculated from sequence | Bifidobacterium bifidum |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bifidobacterium bifidum | Q6JV24 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant | Bifidobacterium bifidum |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2'-L-fucosyllactose + H2O | reaction with recombinant Fuc domain | Bifidobacterium bifidum | L-fucose + lactose | - |
? | |
blood group substance H + H2O | 74% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain | Bifidobacterium bifidum | ? | - |
? | |
lacto-N-fucopentaose I + H2O | 61% of the activity with 2'-fucosyllactose, reaction with recombinant Fuc domain | Bifidobacterium bifidum | ? | - |
? | |
additional information | the recombinant Fuc domain shows no activity with 3-fucosyllactose, lacto-N-fucopentaose II, lacto-N-fucopentaose V and blood group substance B | Bifidobacterium bifidum | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 205000, calculated from sequence | Bifidobacterium bifidum |
Synonyms | Comment | Organism |
---|---|---|
AfcA | - |
Bifidobacterium bifidum |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
35 | - |
30 min, Fuc domain is stable below | Bifidobacterium bifidum |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6.5 | 7.5 | Fuc domain is stable for 12 h | Bifidobacterium bifidum |