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Literature summary for 3.2.1.63 extracted from
- Enzymes that destroy blood group specificity. I. Purification and properties of alpha-L-fucosidase from Clostridium perfringens (1970), J. Biol. Chem., 245, 1659-1669.
General Stability
| General Stability | Organism |
|---|---|
| dialysis, water: rapid loss of activity, buffers: precipitation with considerable inactivation below pH 5.5 | Clostridium perfringens |
| lyophilization causes inactivation | Clostridium perfringens |
| repeated freezing and thawing causes inactivation | Clostridium perfringens |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Cu2+ | - |
Clostridium perfringens |  |
| EDTA | - |
Clostridium perfringens | |
| Fe2+ | - |
Clostridium perfringens | |
| Fe3+ | - |
Clostridium perfringens | |
| Hg2+ | - |
Clostridium perfringens | |
| iodoacetamide | - |
Clostridium perfringens | |
| p-chloromercuribenzoate | - |
Clostridium perfringens | |
| Zn2+ | - |
Clostridium perfringens | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 200000 | - |
value greater than, multiple isozymic forms | Clostridium perfringens |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| submaxillary glycoprotein + H2O | Clostridium perfringens | degradation of blood group substances | L-fucose + ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Clostridium perfringens | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Clostridium perfringens |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 70 | - |
- |
Clostridium perfringens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | not: p-nitrophenyl-alpha-L-fucoside, 2-O-, 3-O- and 4-O-alpha-L-fucopyranosylfucoses, 3-O- and 4-O-alpha-L-fucopyranosyl-D-galactoses, action on oligosaccharides and glycoproteins, not: simple methyl nitrophenyl fucosides | Clostridium perfringens | ? | - |
? | |
| O-alpha-L-fucose-(1-2)-O-beta-D-galactose-(1-4)-D-glucose + H2O | i.e. 2-O-alpha-L-fucosyllactose | Clostridium perfringens | L-fucose + lactose | - |
? | |
| submaxillary glycoprotein + H2O | degradation of blood group substances | Clostridium perfringens | L-fucose + ? | - |
? | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 6 | - |
- |
Clostridium perfringens |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 8 | - |
Clostridium perfringens |
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Release 2023.1 (January 2023)
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