Inhibitors | Comment | Organism | Structure |
---|---|---|---|
EDTA | 1 mM, 82% inhibition | Bacillus sp. (in: Bacteria) | |
Hg2+ | 1 mM HgCl2, complete inhibition | Bacillus sp. (in: Bacteria) | |
phenylmethylsulfonyl fluoride | 1 mM, 4°C, 10 h incubation, 76% inhibition | Bacillus sp. (in: Bacteria) |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Bacillus sp. (in: Bacteria) | - |
- |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM AgNO3 activates | Bacillus sp. (in: Bacteria) | |
Ca2+ | 1 mM CaCl2 activates | Bacillus sp. (in: Bacteria) | |
Co2+ | 1 mM CoCl2 activates | Bacillus sp. (in: Bacteria) | |
Cu2+ | 1 mM CuCl2 activates | Bacillus sp. (in: Bacteria) | |
Mg2+ | 1 mM MgCl2 activates | Bacillus sp. (in: Bacteria) |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
97000 | - |
SDS-PAGE | Bacillus sp. (in: Bacteria) |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bacillus sp. (in: Bacteria) | five alkaline amylases are produced in culture broth | ? | - |
? | |
additional information | Bacillus sp. (in: Bacteria) GM8901 | five alkaline amylases are produced in culture broth | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bacillus sp. (in: Bacteria) | - |
GM8901 | - |
Bacillus sp. (in: Bacteria) GM8901 | - |
GM8901 | - |
Purification (Comment) | Organism |
---|---|
- |
Bacillus sp. (in: Bacteria) |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
(Glcalpha(1-4))n + H2O = (Glcalpha(1-4))n-4 + (Glcalpha(1-4))4 | exo-cleavage mechanism | Bacillus sp. (in: Bacteria) |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
158 | - |
- |
Bacillus sp. (in: Bacteria) |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | - |
Bacillus sp. (in: Bacteria) | maltotetraose + ? | - |
? | |
amylose + H2O | - |
Bacillus sp. (in: Bacteria) | maltotetraose | - |
? | |
amylose + H2O | - |
Bacillus sp. (in: Bacteria) GM8901 | maltotetraose | - |
? | |
maltoheptaose + H2O | - |
Bacillus sp. (in: Bacteria) | maltotetraose + maltotriose | - |
? | |
maltohexaose + H2O | - |
Bacillus sp. (in: Bacteria) | maltotetraose + maltose | - |
? | |
maltohexaose + H2O | - |
Bacillus sp. (in: Bacteria) GM8901 | maltotetraose + maltose | - |
? | |
maltopentaose + H2O | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
maltopentaose + H2O | - |
Bacillus sp. (in: Bacteria) GM8901 | ? | - |
? | |
additional information | five alkaline amylases are produced in culture broth | Bacillus sp. (in: Bacteria) | ? | - |
? | |
additional information | five alkaline amylases are produced in culture broth | Bacillus sp. (in: Bacteria) GM8901 | ? | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) | maltotetraose | - |
? | |
starch + H2O | - |
Bacillus sp. (in: Bacteria) GM8901 | maltotetraose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | 80 | 30°C, 40% of maximal activity, 80°C, 20% of maximal activity | Bacillus sp. (in: Bacteria) |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
1 mM CaCl2 and 1% soluble starch enhance thermostability | Bacillus sp. (in: Bacteria) |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
10.5 | 12 | - |
Bacillus sp. (in: Bacteria) |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 13 | pH 6.0, 30%, pH 13.0, 55% of maximal activity | Bacillus sp. (in: Bacteria) |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | 13 | stable between, 85-100% of maximal activity | Bacillus sp. (in: Bacteria) |