Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Pelomonas saccharophila |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | AgNO3, recombinant and native enzyme | Pelomonas saccharophila | |
Cu2+ | CuSO4, recombinant and native enzyme | Pelomonas saccharophila | |
Fe3+ | FeCl3, recombinant and native enzyme | Pelomonas saccharophila | |
Pb2+ | Pb(OAc)2, recombinant and native enzyme | Pelomonas saccharophila | |
Zn2+ | - |
Pelomonas saccharophila |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
56000 | - |
recombinant and native enzyme, SDS-PAGE | Pelomonas saccharophila |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pelomonas saccharophila | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
amylopectin + H2O | recombinant and native enzyme | Pelomonas saccharophila | maltotetraose + ? | - |
? | |
amylose + H2O | recombinant and native enzyme | Pelomonas saccharophila | maltotetraose | - |
? | |
glycogen + H2O | recombinant and native enzyme, oyster and rabbit liver glycogen | Pelomonas saccharophila | maltotetraose | - |
? | |
starch + H2O | recombinant and native enzyme | Pelomonas saccharophila | maltotetraose | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
recombinant and native enzyme | Pelomonas saccharophila |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
additional information | - |
2 mM CaCl2, thermal stability of the recombinant and native enzymes increase | Pelomonas saccharophila |
40 | - |
recombinant and native enzyme stable on heating in 10 mM acetate buffer, pH 6.0, 30 min, 40°C and below | Pelomonas saccharophila |
60 | - |
recombinant and native enzyme, complete loss of activity | Pelomonas saccharophila |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.5 | - |
recombinant and native enzyme | Pelomonas saccharophila |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 9 | recombinant and native enzyme, pH 4.0, 30%, pH 9.0, 50% of maximal activity | Pelomonas saccharophila |