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Literature summary for 3.2.1.60 extracted from
- Purification of alpha-maltotetraose-forming exo-amylase of Pseudomonas stutzeri-two forms of the amylase and their enzymatic properties (1983), Agric. Biol. Chem., 47, 1761-1768.
No PubMed abstract available
General Stability
| General Stability | Organism |
|---|---|
| Ca2+ stabilizes | Pseudomonas stutzeri |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Al3+ | F1 and F2 form 30% inhibition | Pseudomonas stutzeri |  |
| Co2+ | F1 and F2 form 47% inhibition | Pseudomonas stutzeri | |
| Cu2+ | F1 and F2 form 92% inhibition | Pseudomonas stutzeri | |
| Fe2+ | F1 form, 96% F2 form, 98% inhibition | Pseudomonas stutzeri | |
| Fe3+ | F1 form, 75% F2 form, 76% inhibition | Pseudomonas stutzeri | |
| Hg2+ | F1 and F2 form, complete inhibition | Pseudomonas stutzeri | |
| N-bromosuccinimide | F1 form, pH 6.5 complete inhibition, pH 8.0, 93% inhibition, F2 form, pH 6.5, 95% inhibition, pH 8.0, 28% inhibition | Pseudomonas stutzeri | |
| Ni2+ | F1 form 62%, F2 form 67% inhibition | Pseudomonas stutzeri | |
| Zn2+ | F1 form 94%, F2 form 96% inhibition | Pseudomonas stutzeri | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.32 | - |
amylose | F1 and F2 form | Pseudomonas stutzeri | |
| 5.7 | - |
starch | F2 form | Pseudomonas stutzeri | |
| 5.9 | - |
starch | shellfish glycogen, F1 and F2 form | Pseudomonas stutzeri | |
| 5.9 | - |
starch | F1 form | Pseudomonas stutzeri | |
| 6.7 | - |
oyster glycogen | F2 form | Pseudomonas stutzeri | |
| 7.1 | - |
oyster glycogen | F1form | Pseudomonas stutzeri |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 55000 | - |
F1 and F2 form, SDS-PAGE | Pseudomonas stutzeri |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pseudomonas stutzeri | - |
NRRL B-3389 | - |
| Pseudomonas stutzeri NRRL B-3389 | - |
NRRL B-3389 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| two active forms, F1 and F2 | Pseudomonas stutzeri |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 102 | - |
F2 form | Pseudomonas stutzeri |
| 104 | - |
F1 form | Pseudomonas stutzeri |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| amylopectin + H2O | - |
Pseudomonas stutzeri | maltotetraose + ? | - |
? | |
| amylopectin + H2O | - |
Pseudomonas stutzeri NRRL B-3389 | maltotetraose + ? | - |
? | |
| amylose + H2O | - |
Pseudomonas stutzeri | maltotetraose | - |
? | |
| amylose + H2O | - |
Pseudomonas stutzeri NRRL B-3389 | maltotetraose | - |
? | |
| glycogen + H2O | oyster and shellfish glycogen | Pseudomonas stutzeri | maltotetraose | - |
? | |
| glycogen + H2O | oyster and shellfish glycogen | Pseudomonas stutzeri NRRL B-3389 | maltotetraose | - |
? | |
| oyster glycogen + H2O | - |
Pseudomonas stutzeri | maltotetraose + ? | - |
? | |
| oyster glycogen + H2O | - |
Pseudomonas stutzeri NRRL B-3389 | maltotetraose + ? | - |
? | |
| starch + H2O | - |
Pseudomonas stutzeri | maltotetraose | - |
? | |
| starch + H2O | - |
Pseudomonas stutzeri NRRL B-3389 | maltotetraose | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 45 | - |
pH 8.0, F1 and F2 form | Pseudomonas stutzeri |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | 65 | F1 and F2 form 25°C, 60% of maximal activity, 65°C, 20% of maximal activity | Pseudomonas stutzeri |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
F1 and F2 form, stable below on the treatment at pH 8.0, 60 min in the presence or absence of 5 mM CaCl2 | Pseudomonas stutzeri |
| 55 | - |
F1 and F2 form, complete loss of activity in the absence of Ca2+ | Pseudomonas stutzeri |
| 60 | - |
F1 and F2 form, complete loss of activity in the presence of Ca2+ | Pseudomonas stutzeri |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | 8.2 | F1 and F2 form | Pseudomonas stutzeri |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | 11 | 30°C, 60 min, presence of CaCl2, stable between | Pseudomonas stutzeri |
| 6.5 | 8.5 | absence of Ca2+, 30°C, 60 min stable between | Pseudomonas stutzeri |
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