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Literature summary for 3.2.1.59 extracted from

  • Villalobos-Duno, H.; San-Blas, G.; Paulinkevicius, M.; Sanchez-Martin, Y.; Nino-Vega, G.
    Biochemical characterization of Paracoccidioides brasiliensis beta-1,3-glucanase Agn1p, and its functionality by heterologous expression in Schizosaccharomyces pombe (2013), PLoS ONE, 8, e66853.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 3.2.1.59

Cloned(Commentary)

Cloned (Comment) Organism
gene agn1, sequence comparisons and phylogenetic analysis, heterologous expression in Schizosaccharomyces pombe agn1 null mutant strain 1252. Complementation of a Schizosaccharomyces pombe agn1DELTA strain with the Paracoccidioides brasiliensis AGN1 gene restores the wild-type phenotype, indicating functionality of the gene Paracoccidioides brasiliensis

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition by 1-deoxynojirimycin and D-glucono-1,5-lactone Paracoccidioides brasiliensis

Organism

Organism UniProt Comment Textmining
Paracoccidioides brasiliensis A7L6C9 gene agn1
-
Paracoccidioides brasiliensis Pb73 A7L6C9 gene agn1
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
carboxymethyl alpha-1,3-glucan + H2O the enzyme displays an endolytic cut pattern and high specificity for the soluble substrate, chemically modified from Paracoccidioides brasiliensis alpha-1,3-glucan Paracoccidioides brasiliensis ?
-
 ?
carboxymethyl alpha-1,3-glucan + H2O the enzyme displays an endolytic cut pattern and high specificity for the soluble substrate, chemically modified from Paracoccidioides brasiliensis alpha-1,3-glucan Paracoccidioides brasiliensis Pb73 ?
-
 ?
additional information no activity with starch, carboxymethyl-chitin, carboxymethyl-laminarin, carboxy-methylcellulose, and dextran T500 Paracoccidioides brasiliensis ?
-
 ?
additional information no activity with starch, carboxymethyl-chitin, carboxymethyl-laminarin, carboxy-methylcellulose, and dextran T500 Paracoccidioides brasiliensis Pb73 ?
-
 ?

Synonyms

Synonyms Comment Organism
Agn1p
-
 Paracoccidioides brasiliensis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
40
-
-
 Paracoccidioides brasiliensis

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
23 50 activity range Paracoccidioides brasiliensis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
 Paracoccidioides brasiliensis

pH Range

pH Minimum pH Maximum Comment Organism
4 7.2 activity range Paracoccidioides brasiliensis

General Information

General Information Comment Organism
evolution the enzyme belongs to the glycosyl hydrolase family 71, GH71, phylogenetic tree, overview Paracoccidioides brasiliensis
physiological function possible role of the enzyme in the remodeling of Paracoccidioides brasiliensis Y phase cell wall Paracoccidioides brasiliensis