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Literature summary for 3.2.1.59 extracted from
- Selection of strain and optimization of mutanase production in submerged cultures of Trichoderma harzianum (2002), Acta Biol. Hung., 53, 389-401.
Application
| Application | Comment | Organism |
|---|---|---|
| medicine | the enzyme has a potential as a caries preventive agent, due to the ability to remove the biofilms created by oral bacteria in vitro and to reduce plaque formation in vivo | Trichoderma harzianum |
General Stability
| General Stability | Organism |
|---|---|
| the enzyme in crude state is highly stable after two treatments of freezing and thawing | Trichoderma harzianum |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| extracellular | - |
Aspergillus nidulans | - |
- |
| extracellular | - |
Trichoderma harzianum | - |
- |
| extracellular | - |
Trichoderma reesei | - |
- |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Aspergillus nidulans | - |
- |
- |
| Aspergillus nidulans 1 | - |
- |
- |
| Trichoderma harzianum | - |
strain F-470 is a highly effective producer of mutanase. Effective production by strain f340. Low production by strains 55A5, strain 70B5, strain 32A3 and strain 53059 | - |
| Trichoderma reesei | - |
effective production by strain M-7, low production by strain strain F-560 | - |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| cell culture | submerged | Aspergillus nidulans | - |
| cell culture | submerged | Trichoderma harzianum | - |
| cell culture | submerged | Trichoderma reesei | - |
Storage Stability
| Storage Stability | Organism |
|---|---|
| 4°C, crude enzyme is stable for about 2 weeks | Trichoderma harzianum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| mutan + H2O | - |
Aspergillus nidulans | ? | - |
? |  |
| mutan + H2O | - |
Trichoderma harzianum | ? | - |
? | |
| mutan + H2O | - |
Trichoderma reesei | ? | - |
? | |
| mutan + H2O | - |
Aspergillus nidulans 1 | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 40 | - |
- |
Trichoderma harzianum |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | 50 | 30°C: about 75% of maximal activity, 50°C: about 30% of maximal activity | Trichoderma harzianum |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 21 | - |
diluted enzyme, 1:19, 0.2 M acetate bufferm pH 5.5, at room temperature is stable for 24-48 h | Trichoderma harzianum |
| 35 | - |
1 h, stable up to | Trichoderma harzianum |
| 45 | - |
1 h, enzyme loses 23% of its maximal activity | Trichoderma harzianum |
| 60 | - |
1 h, complete loss of activity | Trichoderma harzianum |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5.5 | - |
- |
Trichoderma harzianum |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 7 | 4.5: about 35% of maximal activity, pH 7.0: about 60% of maximal activity | Trichoderma harzianum |
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