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Literature summary for 3.2.1.59 extracted from

  • Meyer, M.T.; Phaff, H.J.
    Purification and properties of (1->3)-alpha-glucanasea from Bacillus circulans WL-12 (1980), J. Gen. Microbiol., 118, 197-208.
No PubMed abstract available
Search for more literature for 3.2.1.59

Activating Compound

Activating Compound Comment Organism Structure
EDTA
-
 Niallia circulans

General Stability

General Stability Organism
freezing, -15°C, appreciable loss in activity Niallia circulans

Inhibitors

Inhibitors Comment Organism Structure
Ag+
-
 Niallia circulans
Cu2+
-
 Niallia circulans
Hg2+
-
 Niallia circulans
iodoacetamide
-
 Niallia circulans
Mn2+
-
 Niallia circulans
Pb2+
-
 Niallia circulans
Zn2+
-
 Niallia circulans

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information
-
 Niallia circulans

Localization

Localization Comment Organism GeneOntology No. Textmining
extracellular
-
 Niallia circulans
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ no effect Niallia circulans
Co2+ no effect Niallia circulans
additional information no requirement of divalent metal Niallia circulans

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
134000
-
 SDS-PAGE Niallia circulans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
glucan + H2O Niallia circulans
-
 ?
-
 ?
isolichenin + H2O Niallia circulans
-
 ?
-
 ?
nigeran + H2O Niallia circulans
-
 ?
-
 ?
pseudonigeran + H2O Niallia circulans
-
 ?
-
 ?

Organism

Organism UniProt Comment Textmining
Niallia circulans
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
no glycoprotein
-
 Niallia circulans

Purification (Commentary)

Purification (Comment) Organism
-
 Niallia circulans

Source Tissue

Source Tissue Comment Organism Textmining
culture filtrate
-
 Niallia circulans
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.52
-
-
 Niallia circulans
11.2
-
-
 Niallia circulans

Storage Stability

Storage Stability Organism
4°C, 2 years, pH 6, stable Niallia circulans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
glucan + H2O
-
 Niallia circulans ?
-
 ?
glucan + H2O alpha-1,3-glucan Niallia circulans nigerose + glucose
-
 ?
isolichenin + H2O
-
 Niallia circulans ?
-
 ?
additional information
-
 Niallia circulans ?
-
 ?
nigeran + H2O
-
 Niallia circulans ?
-
 ?
pseudonigeran + H2O
-
 Niallia circulans ?
-
 ?

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
40
-
 5% loss of activity after 90 min Niallia circulans
45
-
 25% loss of activity after 90 min Niallia circulans
50
-
 complete loss of activity after 20 min Niallia circulans

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5 8.5
-
 Niallia circulans

pH Stability

pH Stability pH Stability Maximum Comment Organism
3.8
-
 50% loss of activity after 10 min Niallia circulans
4.2
-
 50% loss of activity after 30 min Niallia circulans
5.25 10.5 30°C, stable for at least 1.5 h Niallia circulans