Literature summary for 3.2.1.56 extracted from

Gu, K.; Linhardt, R.L.; Laliberte, M.; Gu, K.; Zimmermann, J.
Purification, characterization and specificity of chondroitin lyases and glycuronidase from Flavobacterium heparinum (1995), Biochem. J., 312, 569-577.

Search for more literature for 3.2.1.56

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
163	-	3-O-(4-deoxy-alpha-L-threo-4-enuronic acid)-(2-N-acetylgalactosamine-6-O-sulfate)	-	Pedobacter heparinus

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
41800	-	SDS-PAGE	Pedobacter heparinus

Organism

Organism	UniProt	Comment	Textmining
Pedobacter heparinus	-	gram-negative soil bacterium, also known as Cythophaga heparinia	-

Purification (Commentary)

Purification (Comment)	Organism
purified by 639fold to apparent homogeneity	Pedobacter heparinus

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
29.4	-	gel filtration	Pedobacter heparinus

Storage Stability

Storage Stability	Organism
-70°C	Pedobacter heparinus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3-O-(4-deoxy-alpha-L-threo-4-enuronic acid)-(2-N-acetylgalactosamine-6-O-sulfate) + H2O	-	Pedobacter heparinus	4-deoxy-beta-L-threo-hex-4-enopyranuronic acid + 2-deoxy-2-acetamido-D-galactopyranose 6-sulfate	-	?
O-3-D-glucuronosyl-N2,O6-disulfo-beta-D-glucosamine + H2O	-	Pedobacter heparinus	N2,O6-disulfo-D-glucosamine + D-glucuronate	-	?

Synonyms

Synonyms	Comment	Organism
chondro-1-3-glycuronidase	hydrolyses 1,3-linked unsaturated disaccharides	Pedobacter heparinus
chondroglycuronidase	-	Pedobacter heparinus
heparo-glycuronidase	hydrolyses 1,4-linked disaccharides, derived from heparin and heparan sulfate	Pedobacter heparinus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	activity decrease of 50% after 3.2 h	Pedobacter heparinus

Temperature Range [°C]

Temperature Minimum [°C]	Temperature Maximum [°C]	Comment	Organism
10	60	-	Pedobacter heparinus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
6.5	-	-	Pedobacter heparinus

pH Range

pH Minimum	pH Maximum	Comment	Organism
4.5	9	-	Pedobacter heparinus

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Release 2023.1 (January 2023)