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Literature summary for 3.2.1.55 extracted from

  • Xie, J.; Zhao, D.; Zhao, L.; Pei, J.; Xiao, W.; Ding, G.; Wang, Z.; Xu, J.
    Characterization of a novel arabinose-tolerant alpha-L-arabinofuranosidase with high ginsenoside Rc to ginsenoside Rd bioconversion productivity (2016), J. Appl. Microbiol., 120, 647-660.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression in Escherichia coli Pseudothermotoga thermarum

Inhibitors

Inhibitors Comment Organism Structure
Hg2+ 1 mM, no residual activtiy Pseudothermotoga thermarum
additional information not inhibitory at 1 mM: EDTA Pseudothermotoga thermarum

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.186
-
4-nitrophenyl alpha-L-arabinofuranoside pH 5.0, 95°C Pseudothermotoga thermarum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
55000
-
x * 55000, SDS-PAGE Pseudothermotoga thermarum

Organic Solvent Stability

Organic Solvent Comment Organism
DMSO 10% v/v, 69% residual activity Pseudothermotoga thermarum
Ethanol 10% v/v, 71% residual activity Pseudothermotoga thermarum
Methanol 10% v/v, 56% residual activity Pseudothermotoga thermarum

Organism

Organism UniProt Comment Textmining
Pseudothermotoga thermarum F7YTI3
-
-
Pseudothermotoga thermarum DSM 5069 F7YTI3
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Pseudothermotoga thermarum 4-nitrophenol + L-arabinose
-
?
4-nitrophenyl alpha-L-arabinofuranoside + H2O
-
Pseudothermotoga thermarum DSM 5069 4-nitrophenol + L-arabinose
-
?
ginsenoside Rc + H2O
-
Pseudothermotoga thermarum ginsenoside Rd + L-arabinofuranose
-
?
ginsenoside Rc + H2O
-
Pseudothermotoga thermarum DSM 5069 ginsenoside Rd + L-arabinofuranose
-
?

Subunits

Subunits Comment Organism
? x * 55000, SDS-PAGE Pseudothermotoga thermarum

Synonyms

Synonyms Comment Organism
Theth_1120
-
Pseudothermotoga thermarum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
95
-
-
Pseudothermotoga thermarum

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
75 100 more than 50% of maximum activtiy Pseudothermotoga thermarum

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
151
-
4-nitrophenyl alpha-L-arabinofuranoside pH 5.0, 95°C Pseudothermotoga thermarum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
5
-
-
Pseudothermotoga thermarum

pH Stability

pH Stability pH Stability Maximum Comment Organism
4 7.5 stable Pseudothermotoga thermarum

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
811
-
4-nitrophenyl alpha-L-arabinofuranoside pH 5.0, 95°C Pseudothermotoga thermarum